Effect of kisspeptin-54 on ovarian levels of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in an experimental model of ovarian hyperstimulation syndrome (OHSS)
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2021
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The purpose of this study was to investigate the effect of kisspeptin-54 on ovarian morphology and vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), protein kinase A (PKA) and protein kinase C (PKC) levels in an ovarian hyperstimulation syndrome (OHSS) rat model, which is a possible complication of controlled ovarian hyperstimulation. For this purpose, immature female Sprague-Dawley rats (25 days old, 30-40 g) were randomly divided into five groups (control, sham, OHSS model, short-term kisspeptin-54 administered OHSS model and long-term kisspeptin-54 administered OHSS model). Serum LH and FSH levels were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and quantitative RT-PCR were performed for VEGF, PEDF, PKA and PKC in ovaries and granulosa cells, respectively. It was observed that there was dilatation in fallopian tubes and an abnormal increase in ovarian weight and volume in the OHSS group, and these morphologies decreased with kisspeptin-54 treatment. After the administration of kisspeptin-54 in the OHSS group, VEGF, PKA and PKC levels reduced and PEDF levels increased in both mRNA and protein levels. It was determined that in the OHSS model, VEGF increased as PEDF decreased, and kisspeptin-54 reduced the effects of OHSS. It was determined that long-term kisspeptin-54 treatment was more effective than short-term administration. It is considered that kisspeptin-54 is an agent that protects ovarian reserve and oocyte maturation in women at risk of OHSS. © 20212021TheAuthor(s)(ortheiremployer(s)).PublishedbyCSIROPublishing.; RD21140_toc.jpg © 2021
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Animals , Disease Models, Animal , Eye Proteins , Female , Kisspeptins , Nerve Growth Factors , Ovarian Hyperstimulation Syndrome , Ovary , Rats , Rats, Sprague-Dawley , Serpins , Vascular Endothelial Growth Factor A , cyclic AMP dependent protein kinase , messenger RNA , metastin , pigment epithelium derived factor , protein kinase C , vasculotropin , eye protein , Kiss1 protein, rat , kisspeptin , nerve growth factor , pigment epithelium-derived factor , serine proteinase inhibitor , vasculotropin A , adult , animal experiment , animal model , animal tissue , Article , controlled study , enzyme linked immunosorbent assay , Fallopian tube , female , gene expression level , granulosa cell , hormone determination , immunohistochemistry , immunoreactivity , long term care , luteinizing hormone blood level , nonhuman , oocyte maturation , ovarian reserve , ovary function , ovary hyperstimulation , rat , real time polymerase chain reaction , animal , disease model , drug effect , metabolism , ovary , ovary hyperstimulation , Sprague Dawley rat