Evaluation of the performance of LED fluorescent microscopy in diagnosis of tuberculosis; [Tüberküloz tanısında LED floresan mikroskopi performansının değerlendirilmesi]

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dc.contributor.authorErdal G.
dc.contributor.authorSürücüoğlu S.
dc.contributor.authorÖzkütük N.
dc.date.accessioned2024-07-22T08:04:59Z
dc.date.available2024-07-22T08:04:59Z
dc.date.issued2022
dc.description.abstractObjective: The aim of the study is to compare of the results of LED fluorescent microscopy (LED FM) and Ehrlich Ziehl Neelsen (EZN) staining in the examination of the respiratory specimens and to evaluate the performance of LED fluorescent microscopy with culture. Methods: In the study, the respiratory specimens obtained from patients prediagnosed with pulmonary tuberculosis were examined in the Tuberculosis Laboratory of Manisa Celal Bayar University Hafsa Sultan Hospital between February 2018 and November 2019. Two smears from each specimen processed with NALC-NaOH method were prepared and they were evaluated both EZN and LED FM by blind readers. The results of culture were used as reference for determination of the performance of LED FM. Results: In the study 1499 respiratory specimens were evaluated with LED FM. Mycobacteria were grown in the culture of 134 specimens (8.9%). The sensitivity, specificity, positive predictive value, and negative predictive value of LED FM were found as 64.2% (95% GI, 61.8%-64.4%), 96% (95%GI, 95.7%-96.3%), 61.4% and 96.5%, respectively. The sensitivity, specificity, positive and negative predictive values of EZN were also found as 51.5% (95% GI, 49%-54%), 99.9% (95% GI, 99.7%-99.9%), 97.2% and 95.4%, respectively. However, smear scarce-culture negative results (54/1365) with LED FM were obtained significantly higher than the results with EZN (0/1365, p=0.000). The reason of this was thought to be due to the lack of the experience of the readers. The reading time of LED FM was 69% time saving. The consistency between the readers was found to be good (Kappa value=0.71) and it was determined that LED FM could be preferred in routine examination as a result of the survey conducted to the readers. Conclusion: The sensitivity of LED FM was found 12.7% higher than the sensitivity of EZN. However, because of the high rate of false positive results, the introduction of LED-FM should be accompanied by appropriate training of the readers. It is also thought that the use of LED FM will be cost-effective in large-scale laboratories in our country. © 2022,Turk Hijyen ve Deneysel Biyoloji Dergisi,All rights reserved
dc.identifier.DOI-ID10.5505/TurkHijyen.2022.46244
dc.identifier.issn03779777
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12939
dc.language.isoEnglish
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.rightsAll Open Access; Bronze Open Access
dc.titleEvaluation of the performance of LED fluorescent microscopy in diagnosis of tuberculosis; [Tüberküloz tanısında LED floresan mikroskopi performansının değerlendirilmesi]
dc.typeArticle

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