Increased expression of VEGF, iNOS, IL-1β, and IL-17 in a rabbit model of gastric content-induced middle ear inflammation

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dc.contributor.authorBaşoĝlu M.S.
dc.contributor.authorEren E.
dc.contributor.authorAslan H.
dc.contributor.authorKolatan H.E.
dc.contributor.authorÖzbay C.
dc.contributor.authorÏnan S.
dc.contributor.authorKaraca F.
dc.contributor.authorÖztürkcan S.
dc.contributor.authorKatilmiş H.
dc.date.accessioned2024-07-22T08:19:54Z
dc.date.available2024-07-22T08:19:54Z
dc.date.issued2012
dc.description.abstractObjective: To investigate the histopathological changes and the expression of vascular endothelial growth factor (VEGF), inducible NO-synthase (iNOS), endothelial NO-synthase (eNOS), interleukin (IL)-1β, and IL-17 in the rabbit middle ear mucosa after direct gastric content exposure. Methods: Exploratory controlled study in which histological and immunochemical features were studied after gastric content-induced inflammation was established in rabbits. Sixteen healthy rabbits were divided into two equal groups. Gastric contents of an animal were injected into the middle ear of the same animal for 20 days. Saline was injected into the middle ear of the animals in the control group. The rabbits were humanely killed on day 27. Inflammation was assayed by light microscopy. Immunochemical staining was performed for VEGF, iNOS, eNOS, IL-1β, and IL-17 expression. Experimental and control animals were examined using the same protocol. Results: The expression levels of VEGF, iNOS, IL-1β, and IL-17 differed significantly between the experimental and control groups (p=0.018, p=0.010, p=0.002, and p=0.002, respectively). The expression level of eNOS was not significantly different between the two groups (p=0.132). Conclusion: This study demonstrates that gastroesophagial reflux induced middle ear inflammation is associated with increased expression of VEGF, IL-1β, IL-17, and iNOS. © 2011 Elsevier Ireland Ltd.
dc.identifier.DOI-ID10.1016/j.ijporl.2011.10.001
dc.identifier.issn18728464
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17873
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectBiological Markers
dc.subjectBiopsy, Needle
dc.subjectDisease Models, Animal
dc.subjectGastric Juice
dc.subjectImmunohistochemistry
dc.subjectInterleukin-17
dc.subjectInterleukin-1beta
dc.subjectMale
dc.subjectNitric Oxide Synthase Type II
dc.subjectOtitis Media
dc.subjectRabbits
dc.subjectRandom Allocation
dc.subjectSensitivity and Specificity
dc.subjectVascular Endothelial Growth Factor A
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 17
dc.subjectinterleukin 1beta
dc.subjectsodium chloride
dc.subjectvasculotropin
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdisease association
dc.subjectexperimental rabbit
dc.subjectgastroesophageal reflux
dc.subjecthistopathology
dc.subjectimmunochemistry
dc.subjectnonhuman
dc.subjectotitis media
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectstomach content
dc.titleIncreased expression of VEGF, iNOS, IL-1β, and IL-17 in a rabbit model of gastric content-induced middle ear inflammation
dc.typeArticle

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