The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction

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dc.contributor.authorOzdal-Kurt F.
dc.contributor.authorTuʇlu I.
dc.contributor.authorVatansever H.
dc.contributor.authorTong S.
dc.contributor.authorDeliloʇlu-Gurhan S.
dc.date.accessioned2024-07-22T08:12:57Z
dc.date.available2024-07-22T08:12:57Z
dc.date.issued2015
dc.description.abstractBone marrow contains mesenchymal stem cells that form many tissues. Various scaffolds are available for bone reconstruction by tissue engineering. Osteoblastic differentiated bone marrow stromal cells (BMSC) promote osteogenesis on scaffolds and stimulate bone regeneration. We investigated the use of cultured autologous BMSC on different scaffolds for healing defects in tibias of adult male canines. BMSC were isolated from canine humerus bone marrow, differentiated into osteoblasts in culture and loaded onto porous ceramic scaffolds including hydroxyapatite 1, hydroxyapatite gel and calcium phosphate. Osteoblast differentiation was verified by osteonectine and osteocalcine immunocytochemistry. The scaffolds with stromal cells were implanted in the tibial defect. Scaffolds without stromal cells were used as controls. Sections from the defects were processed for histological, ultrastructural, immunohistochemical and histomorphometric analyses to analyze the healing of the defects. BMSC were spread, allowed to proliferate and differentiate to osteoblasts as shown by alizarin red histochemistry, and osteocalcine and osteonectine immunostaining. Scanning electron microscopy showed that BMSC on the scaffolds were more active and adhesive to the calcium phosphate scaffold compared to the others. Macroscopic bone formation was observed in all groups, but scaffolds with stromal cells produced significantly better results. Bone healing occurred earlier and faster with stromal cells on the calcium phosphate scaffold and produced more callus compared to other scaffolds. Tissue healing and osteoblastic marker expression also were better with stromal cells on the scaffolds. Increased trabecula formation, cell density and decreased fibrosis were observed in the calcium phosphate scaffold with stromal cells. Autologous cultured stromal cells on the scaffolds were useful for healing of canine tibial bone defects. The calcium phosphate scaffold was the best for both cell differentiation in vitro and bone regeneration in vivo. It may be possible to improve healing of bone defects in humans using stem cells from bone marrow. © 2015 The Biological Stain Commission.
dc.identifier.DOI-ID10.3109/10520295.2014.983547
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16234
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd.
dc.subjectAnimals
dc.subjectBone and Bones
dc.subjectBone Marrow Cells
dc.subjectBone Regeneration
dc.subjectCalcium Phosphates
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectDogs
dc.subjectDurapatite
dc.subjectMale
dc.subjectMesenchymal Stromal Cells
dc.subjectOsteoblasts
dc.subjectOsteogenesis
dc.subjectTissue Engineering
dc.subjectWound Healing
dc.subjectcalcium phosphate
dc.subjecthydroxyapatite
dc.subjectanimal
dc.subjectbone
dc.subjectbone development
dc.subjectbone marrow cell
dc.subjectbone regeneration
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectdog
dc.subjectdrug effects
dc.subjectmale
dc.subjectmesenchymal stroma cell
dc.subjectosteoblast
dc.subjectphysiology
dc.subjectprocedures
dc.subjecttissue engineering
dc.subjectwound healing
dc.titleThe effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction
dc.typeArticle

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