Determination of sand fly fauna and molecular detection of Leishmania in sand flies in Antalya Province, Southern Turkey

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dc.contributor.authorArserim S.K.
dc.contributor.authorÇetin H.
dc.contributor.authorKarakuş M.
dc.contributor.authorDemir S.
dc.contributor.authorSer Ö.
dc.contributor.authorTöz S.
dc.contributor.authorBalcioğlu I.C.
dc.contributor.authorÖlgen M.K.
dc.contributor.authorYilmaz B.
dc.contributor.authorÖzbel Y.
dc.date.accessioned2024-07-22T08:05:39Z
dc.date.available2024-07-22T08:05:39Z
dc.date.issued2021
dc.description.abstractVisceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are diseases transmitted by infected female sand flies. Since the eradication of malaria in Turkey, CL is the main vector-borne disease in the country, with more than 2000 cases per year, making it a significant public health problem. The aims of this study were to carry out an entomological survey in Antalya Province, an endemic area for CL in the Mediterranean Region of Turkey, to identify sand fly fauna and to screen female specimens for the presence of Leishmania parasites (Leishmania infantum, L. tropica, L. major, and L. donovani) using molecular analysis. Sand flies were collected in 42 localities of seven districts in Antalya Province using CDC miniature light traps in two different periods, June 2012 and September 2013. The specimens were kept in 96% ethanol until the dissection was done. The head and genitalia of the specimens were cut for preparing individual slides for species identification. The rest of the body of female specimens was kept separately. The specimens were identified at the species level, and 27 pools were generated according to the locations and species for screening the presence of Leishmania. A commercial kit was used for DNA extractions. Real-time and conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region (ITS1) were then performed. In total, 1306 specimens comprising nine species belonging to the Phlebotomus genus were collected in the study region, with Phlebotomus neglectus/syriacus (38.82%) the most abundant, followed by P. alexandri (21.67%) and P. tobbi (20.44%). In the 27 pools, Leishmania infantum DNA was detected in four pools containing P. neglectus/syriacus and one pool containing P. tobbi. In conclusion, the sand fly fauna in the Antalya Province is diverse. The probable vector sand fly species are P. neglectus/syriacus and P. tobbi with high dominance (59.26%), which indicates a high risk of CL transmission. The data presented here may help to shed more light on the transmission cycles of the Leishmania parasite in this CL endemic area. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
dc.identifier.DOI-ID10.1007/s00436-021-07279-5
dc.identifier.issn09320113
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13217
dc.language.isoEnglish
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.subjectAnimals
dc.subjectFemale
dc.subjectInsect Vectors
dc.subjectLeishmania infantum
dc.subjectPhlebotomus
dc.subjectPsychodidae
dc.subjectTurkey
dc.subjectArticle
dc.subjectDNA extraction
dc.subjectfemale
dc.subjectgenital system
dc.subjectLeishmania
dc.subjectLeishmania donovani
dc.subjectLeishmania infantum
dc.subjectLeishmania major
dc.subjectLeishmania tropica
dc.subjectmale
dc.subjectnonhuman
dc.subjectPhlebotominae
dc.subjectPhlebotomus
dc.subjectPhlebotomus alexandri
dc.subjectPhlebotomus neglectus
dc.subjectPhlebotomus syriacus
dc.subjectPhlebotomus tobbi
dc.subjectpolymerase chain reaction
dc.subjectSouthern Europe
dc.subjectspecies identification
dc.subjectTurkey (republic)
dc.subjectanimal
dc.subjectepidemiology
dc.subjectinsect vector
dc.subjectLeishmania infantum
dc.subjectPhlebotomus
dc.subjectPsychodidae
dc.subjectturkey (bird)
dc.titleDetermination of sand fly fauna and molecular detection of Leishmania in sand flies in Antalya Province, Southern Turkey
dc.typeArticle

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