Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis

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dc.contributor.authorOzturk E.A.
dc.contributor.authorManzano-Román R.
dc.contributor.authorSánchez-Ovejero C.
dc.contributor.authorCaner A.
dc.contributor.authorAngın M.
dc.contributor.authorGunduz C.
dc.contributor.authorKaraman Ü.
dc.contributor.authorAltintas N.
dc.contributor.authorBozkaya H.
dc.contributor.authorUnalp O.
dc.contributor.authorDokumcu Z.
dc.contributor.authorDivarci E.
dc.contributor.authorCasulli A.
dc.contributor.authorAltintas N.
dc.contributor.authorSiles-Lucas M.
dc.contributor.authorUnver A.
dc.date.accessioned2024-07-22T08:05:16Z
dc.date.available2024-07-22T08:05:16Z
dc.date.issued2022
dc.description.abstractThe use of serological tests containing multiple immunodominant antigens rather than single antigens have the potential to improve the diagnostic performance in Cystic Echinococcoses (CE) as a complement tool to clear the inconclusive imaging data. Here, we comparatively evaluated the diagnostic value of Hydatid Fluid (HF) and the recently described recombinant multi-epitope antigen DIPOL in IgG-ELISA in a clinically defined cohort of CE patients. The serum samples from 149 CE patients were collected just before surgical or Percutaneous- Aspiration- Injection- Reaspiration (PAIR) procedures. Additionally, serum samples of patients with other parasitic infections (n=49) and healthy individuals (n=21) were also included in the study as controls. To investigate the association between the genotype of the parasite and DIPOL, cyst materials from 20 CE patients were sequenced. In terms of overall sensitivity, HF was higher than DIPOL (82.55%,78.52%, respectively). However, while the sensitivity of HF was higher than DIPOL in patients with active and transitional cysts (83.3%, 75.4%, respectively), sensitivity of DIPOL in inactive cysts was higher compared to HF (95.6%, 78.3%, respectively). The sensitivity of DIPOL depending on cyst stage was statistically significant (P= 0.041). In terms of specificity, DIPOL was found to be better than HF (97.71%, 91.43%, respectively). By genotyping, the majority of 20 patients showed G1 genotype (80%). All patients harboring G3 and G1/G3 cyst genotypes were positive with both antigens, while 87.5% of patients with G1 genotype were seropositive with HF and 75% with DIPOL. The overall sensitivity and high specificity of DIPOL suggest that this recombinant protein containing immunodominant epitopes is a potential substitute for the HF by serological tests for the diagnosis of CE. © 2021
dc.identifier.DOI-ID10.1016/j.actatropica.2021.106208
dc.identifier.issn0001706X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13070
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.rightsAll Open Access; Green Open Access
dc.subjectAnimals
dc.subjectAntibodies, Helminth
dc.subjectAntigens, Helminth
dc.subjectEchinococcosis
dc.subjectEchinococcus granulosus
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectEpitopes
dc.subjectHumans
dc.subjectSensitivity and Specificity
dc.subjectSerologic Tests
dc.subjectEchinococcus granulosus
dc.subjectalbendazole
dc.subjectepitope
dc.subjectrecombinant antigen
dc.subjectrecombinant antigen DIPOL
dc.subjectunclassified drug
dc.subjecthelminth antibody
dc.subjectparasite antigen
dc.subjectantigen
dc.subjectcomparative study
dc.subjectechinococcosis
dc.subjectgenotype
dc.subjectimmunoassay
dc.subjectprotein
dc.subjectserum
dc.subjectadolescent
dc.subjectadult
dc.subjectage distribution
dc.subjectaged
dc.subjectantibody detection
dc.subjectantigen specificity
dc.subjectArticle
dc.subjectbody fluid
dc.subjectcellular immunity
dc.subjectchild
dc.subjectcohort analysis
dc.subjectcomparative study
dc.subjectcomputer assisted tomography
dc.subjectcontrolled study
dc.subjectdiagnostic test accuracy study
dc.subjectechinococcosis
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjectgenotype
dc.subjecthuman
dc.subjecthuman tissue
dc.subjecthumoral immunity
dc.subjecthydatid fluid
dc.subjectimmunoreactivity
dc.subjectinfant
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmiddle aged
dc.subjectnuclear magnetic resonance imaging
dc.subjectpredictive value
dc.subjectproteomics
dc.subjectretrospective study
dc.subjectsensitivity and specificity
dc.subjectTurk (people)
dc.subjectyoung adult
dc.subjectanimal
dc.subjectechinococcosis
dc.subjectEchinococcus granulosus
dc.subjectgenetics
dc.subjectserology
dc.titleComparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis
dc.typeArticle

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