The effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells lines

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dc.contributor.authorÖzgürbüz U.
dc.contributor.authorGencür S.
dc.contributor.authorKurt F.Ö.
dc.contributor.authorÖzkalkanlı M.
dc.contributor.authorVatansever H.S.
dc.date.accessioned2024-07-22T08:08:19Z
dc.date.available2024-07-22T08:08:19Z
dc.date.issued2019
dc.description.abstractOpioids are widely used in the treatment of cancer related pain. They mainly exert their effects on opioid receptors. The most common opioid in the treatment of pain is morphine. Previous studies show that they may have effects on cancer cell behavior. These may include apoptosis, angiogenesis, invasion, inflammation and immune reactions. Tramadol, also an opioid is widely used in the treatment of cancer pain and is not well studied in cancer behavior. We aimed to investigate the effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells. We used Colo320 (ATCC, CCL-220), Colo741 (ECACC, 93052621) and HCT116 (ATCC, CCL-247) colon cancer cell lines. CD133 was considered colon cancer stem cell marker and used to sort CD133+ and CD133− cells by magnetic cell sorting. MTT (mitochondria-targeted therapeutics) technique was used to detect tramadol's cytotoxic effect on cells in the study groups. Cells were treated with 1 mg/kg, 1.5 mg/kg and 2 mg/kg tramadol for 24 h at 37 °C and 5% CO2.Caspase-3, Ki-67, Bcl-2 and VGEF distributions were performed using indirect immunoperoxidase staining for immunohistochemical analysis. The study showed that tramadol has triggering effect on apoptosis in Colo320 colon cancer stem cells. © 2019 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.gene.2019.144030
dc.identifier.issn03781119
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14349
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectApoptosis
dc.subjectColonic Neoplasms
dc.subjectCytotoxins
dc.subjectHCT116 Cells
dc.subjectHumans
dc.subjectNeoplasm Invasiveness
dc.subjectNeoplastic Stem Cells
dc.subjectTramadol
dc.subjectcarbon dioxide
dc.subjectcaspase 3
dc.subjectCD133 antigen
dc.subjectKi 67 antigen
dc.subjectperoxidase
dc.subjectprotein bcl 2
dc.subjecttramadol
dc.subjectvasculotropin
dc.subjectcytotoxin
dc.subjecttramadol
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer stem cell
dc.subjectcell metabolism
dc.subjectcell selection
dc.subjectCOLO 320 cell line
dc.subjectColo741 cell line
dc.subjectcolon carcinoma cell line
dc.subjectconcentration (parameter)
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjectdrug effect
dc.subjectdrug targeting
dc.subjectHCT 116 cell line
dc.subjectimmunohistochemistry
dc.subjectincubation temperature
dc.subjectmitochondrion
dc.subjectpriority journal
dc.subjectcolon tumor
dc.subjecthuman
dc.subjectmetabolism
dc.subjectpathology
dc.subjecttumor invasion
dc.titleThe effects of tramadol on cancer stem cells and metabolic changes in colon carcinoma cells lines
dc.typeArticle

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