Immune responses elicited by the recombinant Erp, HspR, lppX, Mmaa4, and OmpA proteins from Mycobacterium tuberculosis in mice
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Date
2018
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Abstract
Immunogenic potency of the recombinant Erp, HspR, LppX, MmaA4, and OmpA proteins from Mycobacterium tuberculosis (MTB), formulated with Montanide ISA 720 VG adjuvant, was evaluated in BALB/c mice for the first time in this study. The five vaccine formulations, adjuvant, and BCG vaccine were subcutaneously injected into mice, and the sera were collected at days 0, 15, 30, 41, and 66. The humoral and cellular immune responses against vaccine formulations were determined by measuring serum IgG and serum interferon-gamma (IFN-γ) and interleukin-12 (IL-12) levels, respectively. All formulations significantly increased IgG levels post-vaccination. The highest increase in IFN-γ level was provided by MmaA4 formulation. The Erp, HspR, and LppX formulations were as effective as BCG in enhancement of IFN-γ level. The most efficient vaccine boosting the IL-12 level was HspR formulation, especially at day 66. Erp formulation also increased the IL-12 level more than BCG at days 15 and 30. The IL-12 level boosted by MmaA4 formulation was found to be similar to that by BCG. OmpA formulation was inefficient in enhancement of cellular immune responses. This study showed that MmaA4, HspR, and Erp proteins from MTB are successful in eliciting both humoral and cellular immune responses in mice. © 2018 Akadémiai Kiadó, Budapest
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Animals , Antibodies, Bacterial , Bacterial Outer Membrane Proteins , Bacterial Proteins , BCG Vaccine , Cytokines , Female , Heat-Shock Proteins , Immunity, Cellular , Immunity, Humoral , Mannitol , Membrane Proteins , Methyltransferases , Mice , Mice, Inbred BALB C , Mixed Function Oxygenases , Mycobacterium tuberculosis , Oleic Acids , Repressor Proteins , Tuberculosis , Tuberculosis Vaccines , Vaccination , Vaccines, Synthetic , bacterial vaccine , BCG vaccine , Erp protein , gamma interferon , HspR protein , immunoglobulin G , immunological adjuvant , interleukin 12 , LppX protein , MmaA4 protein , outer membrane protein A , recombinant protein , recombinant vaccine , unclassified drug , bacterial protein , bacterium antibody , BCG vaccine , cytokine , ERP protein, Mycobacterium tuberculosis , heat shock protein , HspR protein, bacteria , mannide monooleate , mannitol , membrane protein , methyltransferase , mixed function oxidase , mma4 protein, Mycobacterium tuberculossis , oleic acid , OMPA outer membrane proteins , outer membrane protein , recombinant vaccine , repressor protein , animal experiment , animal model , Article , bacterial strain , bacterium isolate , BCG vaccination , cellular immunity , controlled study , drug formulation , female , human , humoral immunity , immune response , immunoglobulin blood level , mouse , Mycobacterium tuberculosis , nonhuman , protein blood level , tuberculosis , vaccine immunogenicity , animal , Bagg albino mouse , blood , genetics , immunology , microbiology , tuberculosis , vaccination