Immunolocalization of αV, α3 and β1 integrins in the human placenta with pre-eclampsia
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2003
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Abstract
Signs of pre-eclampsia are considered to be caused by maternal endothelial dysfunction due to circulating factors of placental origin. Integrins are a large family of cell surface proteins that serve as receptors involved in cell-cell and cell-matrix interactions during placentation. Therefore, low expression of integrins or the lack of it may be encountered during pre-eclampsia. In the present study, we investigated the immunolocalisation of integrins αV, α3 and β1 in placentas of normal and preeclamptic women. Thirty-two placentas from pre-eclamptic (n = 14) and normotensive (n = 18) women were used. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue specimens, using anti-αV, anti-α3 and anti-β1 antibodies and the indirect immunoperoxidase technique. A semi-quantitative grading system (HSCORE) was used to compare immunohistochemical staining intensities. Distribution patterns of αV, α3 and β1 integrins were detected in cytotrophoblasts and Hofbauer cells in normal and pre-eclamptic placentas. Immunostaining of αV and β1 integrins was slightly decreased in pre-eclamptic samples but α3 integrin immunostaining was similar in pre-eclamptic and normal placentas. Decreased immunostaining of integrins in the cytotrophoblasts may considered to be a structural basis for decreased placental perfusion in pre-eclampsia.
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Antigens, CD29 , Female , Humans , Immunohistochemistry , Integrin alpha3 , Integrin alphaV , Placenta , Pre-Eclampsia , Pregnancy , Antibodies , Enzyme kinetics , Histology , Immunology , alpha3 integrin , alpha5 integrin , beta1 integrin , formaldehyde , integrin , monoclonal antibody , paraffin , peroxidase , unclassified drug , alpha3 integrin , beta1 integrin , CD51 antigen , Immunostainings , adult , article , cell function , clinical article , controlled study , cytotrophoblast , endothelium cell , female , human , human tissue , immunohistochemistry , immunolocalization , placenta , preeclampsia , protein expression , protein family , protein function , protein localization , scoring system , chemistry , cytology , histology , immunology , metabolism , pregnancy , Tissue culture