Determination of histopathological effects and myoglobin, periostin gene-protein expression levels in Danio rerio muscle tissue after acaricide yoksorrun-5EC (hexythiazox) application

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dc.contributor.authorBaşımoğlu Koca Y.
dc.contributor.authorKoca S.
dc.contributor.authorÖztel Z.
dc.contributor.authorBalcan E.
dc.date.accessioned2024-07-22T08:03:41Z
dc.date.available2024-07-22T08:03:41Z
dc.date.issued2023
dc.description.abstractAlthough pesticides are essential agrochemicals to annihilate harmful organisms in agriculture, their uncontrolled use has become an important threat to environmental health. Exposure to pesticides can affect many biological systems including immune system, endocrine system, and nervous system. However, the potential side effects of pesticides to skeletal muscle system remain unclear. Present study has focused on the evaluation of this issue by using an acaricide, yoksorrun-5EC (hexythiazox), in an aquatic model organism, Danio rerio. The histological analyses revealed that increased concentrations of the acaricide cause degradation of skeletal muscle along with increased necrosis and atrophy in myocytes, intercellular edema, and increased infiltrations between perimysium sheaths of muscle fibers. The effects of acaricide on myoglobin and periostin, which are associated with oxygen transport and muscle regeneration, respectively, were investigated at the gene and protein levels. RT-PCR results suggested that high concentration yoksorrun-5EC (hexythiazox) can induce myoglobin and periostin genes. Similar results were also obtained in the protein levels of these genes by western blotting analysis. These results suggested that yoksorrun-5EC (hexythiazox)-dependent disruption of skeletal muscle architecture is closely associated with the expression levels of myoglobin and periostin genes in Danio rerio model. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.DOI-ID10.1080/01480545.2021.2007945
dc.identifier.issn01480545
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12378
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd.
dc.subjectAcaricides
dc.subjectAnimals
dc.subjectMuscle, Skeletal
dc.subjectMyoglobin
dc.subjectPesticides
dc.subjectZebrafish
dc.subjectacaricide
dc.subjectbeta actin
dc.subjectmyoglobin
dc.subjecttranscription factor RUNX2
dc.subjectunclassified drug
dc.subjectyoksorrun 5ec
dc.subject5-(4-chlorophenyl)-N-cyclohexyl-4-methyl-2-oxothiazolidine-3 carboxamide
dc.subjectacaricide
dc.subjectmyoglobin
dc.subjectpesticide
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectaquatic environment
dc.subjectArticle
dc.subjectbody mass
dc.subjectconnective tissue
dc.subjectcontrolled study
dc.subjectendomysium
dc.subjectenvironmental exposure
dc.subjectenvironmental health
dc.subjectglycogen analysis
dc.subjecthistopathology
dc.subjectmolecular cloning
dc.subjectmuscle cell
dc.subjectmuscle fibril
dc.subjectmuscle tissue
dc.subjectnonhuman
dc.subjectoxygen diffusion
dc.subjectperimysium
dc.subjectprotein expression level
dc.subjectskeletal muscle
dc.subjectWestern blotting
dc.subjectzebra fish
dc.subjectanimal
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectzebra fish
dc.titleDetermination of histopathological effects and myoglobin, periostin gene-protein expression levels in Danio rerio muscle tissue after acaricide yoksorrun-5EC (hexythiazox) application
dc.typeArticle

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