Evaluation of the performance of maldi-tof ms and dna sequence analysis in the identification of mycobacteria species

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dc.contributor.authorAkyar I.
dc.contributor.authorÇavuşoğlu C.
dc.contributor.authorAyaş M.
dc.contributor.authorSürücüoğlu S.
dc.contributor.authorİlki A.
dc.contributor.authorKaya D.E.
dc.contributor.authorBeşli Y.
dc.date.accessioned2024-07-22T08:10:09Z
dc.date.available2024-07-22T08:10:09Z
dc.date.issued2018
dc.description.abstractBackground/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC. © TÜBİTAK.
dc.identifier.DOI-ID10.3906/sag-1801-198
dc.identifier.issn13000144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15103
dc.language.isoEnglish
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.rightsAll Open Access; Bronze Open Access
dc.subjectarticle
dc.subjectatypical Mycobacterium
dc.subjectcomparative effectiveness
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectdrug efficacy
dc.subjectmajor clinical study
dc.subjectmatrix assisted laser desorption ionization time of flight mass spectrometry
dc.subjectMycobacterium tuberculosis complex
dc.subjectnonhuman
dc.subjectquality control
dc.titleEvaluation of the performance of maldi-tof ms and dna sequence analysis in the identification of mycobacteria species
dc.typeArticle

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