Therapeutic effects of Lacosamide in a rat model of traumatic brain injury: A histological, biochemical and electroencephalography monitoring study

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dc.contributor.authorMete M.
dc.contributor.authorAlpay S.
dc.contributor.authorAydemir I.
dc.contributor.authorUnsal U.U.
dc.contributor.authorCollu F.
dc.contributor.authorÖzel H.F.
dc.contributor.authorDuransoy Y.K.
dc.contributor.authorKutlu N.
dc.contributor.authorTuglu M.İ.
dc.date.accessioned2024-07-22T08:06:09Z
dc.date.available2024-07-22T08:06:09Z
dc.date.issued2021
dc.description.abstractObjective: Traumatic Brain Injury (TBI) is a major cause of death and disability worldwide, especially in children and young adults. TBI can be classified based on severity, mechanism or other features. Inflammation, apoptosis, oxidative stress, and ischemia are some of the important pathophys-iological mechanisms underlying neuronal loss after TBI. Lacosamide (LCM) is an anticonvulsant compound approved for the adjunctive treatment of partial-onset seizures and neuropathic pain. This study aimed to investigate possible neuroprotective effects of LCM in a rat model of TBI. Material and methods: Twenty-eight adult male, Wistar albino rats were used. The rats were divided into 4 groups. Group 1 was the control group (n=7). Group 2 was the trauma group (n=7) where rats were treated with 100 mg/kg saline intraperitoneally (IP) twice a day. Groups 3 and 4, rats were treated with 6 (group 3, n=7) or 20 (group 4, n=7) mg/kg Lacosamide IP twice a day. For each group, brain samples were collected 72 hours after injury. Brain samples and blood were evaluated with histopathological and biochemical methods. In addition, electroencephalograpy monitoring results were compared. Results: The immunoreactivity of both iNOS and eNOS (oxidative stress markers) were decreased with LCM treatment compared to trauma group. The results were statistically significant (***P<0.001). The treatments of low (56,17±9,69) and high-dose LCM (43,91±9,09) were decreased the distribution of HIF-1α compared to trauma group (P<0.01). The number of apoptotic cells were decreased with LCM treatment the difference between the trauma group and 20mg/kg LCM treated group (9,55±1,02) was statistically significant (***P<0.001). Malondialdehyde level was reduced with LCM treatment. MDA level was significantly higher in trauma group compared to LCM treated groups (***P<0.001). The level of Superoxide dismutase in the trauma group was 1,86 U/ml, whereas it was 36,85 U/ml in 20mg/kg LCM treated group (***P<0.001). Delta strength of EEG in 20mg/kg LCM treated group were similar to control group values after LCM treatment. Conclusion: No existing study has produced results suggesting that different doses of LCM has therapeutic effect against TBI, using EEG recording in addition to histological and biochemical evaluations in rats. © 2021 Elsevier Ltd
dc.identifier.DOI-ID10.1016/j.injury.2021.02.055
dc.identifier.issn00201383
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13386
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectAnimals
dc.subjectAnticonvulsants
dc.subjectBrain Injuries, Traumatic
dc.subjectElectroencephalography
dc.subjectLacosamide
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectendothelial nitric oxide synthase
dc.subjecthypoxia inducible factor 1alpha
dc.subjectinducible nitric oxide synthase
dc.subjectlacosamide
dc.subjectmalonaldehyde
dc.subjectsuperoxide dismutase
dc.subjectanticonvulsive agent
dc.subjectlacosamide
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectapoptosis
dc.subjectArticle
dc.subjectbiochemical analysis
dc.subjectcontrolled study
dc.subjectdose response
dc.subjectelectroencephalography monitoring
dc.subjectgene expression
dc.subjecthistopathology
dc.subjectimmunoreactivity
dc.subjectmale
dc.subjectneuropathic pain
dc.subjectneuroprotection
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectseizure
dc.subjecttherapy effect
dc.subjecttraumatic brain injury
dc.subjectanimal
dc.subjectelectroencephalography
dc.subjectWistar rat
dc.titleTherapeutic effects of Lacosamide in a rat model of traumatic brain injury: A histological, biochemical and electroencephalography monitoring study
dc.typeArticle

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