Neuroprotective effects of bone marrow-derived mesenchymal stem cells and conditioned medium in mechanically injured neuroblastoma cells

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dc.contributor.authorMete M.
dc.contributor.authorAydemir I.
dc.contributor.authorÜnlü Ünsal Ü.
dc.contributor.authorDuransoy Y.K.
dc.contributor.authorTuğlu İ.M.
dc.contributor.authorSelçuki M.
dc.date.accessioned2024-07-22T08:12:18Z
dc.date.available2024-07-22T08:12:18Z
dc.date.issued2016
dc.description.abstractBackground/aim: Bone marrow-derived mesenchymal stem cells (BMSCs) possess self-renewal characteristics that distinguish them from other cell types. Recent studies have focused on the effects of conditioned medium (CM) that includes the extracellular matrix. Here we examined the neuroprotective effects of BMSCs and CM on damaged neuroblastoma cells. Materials and methods: The cells were divided into five groups: 1) healthy controls, 2) damaged cells alone, 3) damaged cells treated with BMSCs, 4) damaged cells treated with CM, and 5) damaged cells treated with both BMSCs and CM. Neuroprotective effects were then evaluated based upon the levels of oxidative stress, antitransforming growth factor β1 (anti-TGFβ1) production, and apoptosis. Results: Significant differences were observed between healthy controls and damaged cells (P < 0.001), as well as between damaged cells and those treated with BMSCs alone (P < 0.05), CM alone (P < 0.05), and both BMSCs and CM in combination (P < 0.01). Among the treated groups, the strongest neuroprotective effects were seen in cells treated with both BMSCs and CM. Conclusion: These results show that both BMSCs and CM exhibit neuroprotective effects in damaged neuroblastoma cells. The strongest benefits were seen following treatment with both BMSCs and CM. © TÜBİTAK.
dc.identifier.DOI-ID10.3906/sag-1507-187
dc.identifier.issn13000144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15991
dc.language.isoEnglish
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.rightsAll Open Access; Bronze Open Access
dc.subjectBone Marrow
dc.subjectBone Marrow Cells
dc.subjectCells, Cultured
dc.subjectCulture Media, Conditioned
dc.subjectMesenchymal Stromal Cells
dc.subjectNeuroblastoma
dc.subjectNeuroprotective Agents
dc.subjectendoglin
dc.subjectendothelial nitric oxide synthase
dc.subjecthydrogen peroxide
dc.subjectinducible nitric oxide synthase
dc.subjectperoxidase
dc.subjecttransforming growth factor beta1
dc.subjectneuroprotective agent
dc.subjectanimal cell
dc.subjectapoptosis
dc.subjectArticle
dc.subjectbone marrow derived mesenchymal stem cell
dc.subjectcancer cell culture
dc.subjectcell damage
dc.subjectcell permeabilization
dc.subjectconditioned medium
dc.subjectcontrolled study
dc.subjectimmunocytochemistry
dc.subjectmale
dc.subjectneuroblastoma cell
dc.subjectneuroprotection
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectprotein expression
dc.subjectrat
dc.subjectstem cell self-renewal
dc.subjectTUNEL assay
dc.subjectbone marrow
dc.subjectbone marrow cell
dc.subjectcell culture
dc.subjectmesenchymal stroma cell
dc.subjectneuroblastoma
dc.titleNeuroprotective effects of bone marrow-derived mesenchymal stem cells and conditioned medium in mechanically injured neuroblastoma cells
dc.typeArticle

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