Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages

Simple item page
dc.contributor.authorEkizceli G.
dc.contributor.authorInan S.
dc.contributor.authorOktem G.
dc.contributor.authorOnur E.
dc.contributor.authorOzbilgin K.
dc.date.accessioned2024-07-22T08:07:54Z
dc.date.available2024-07-22T08:07:54Z
dc.date.issued2020
dc.description.abstractmTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/ mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process. © 2020, Histology and Histopathology. All rights reserved.
dc.identifier.DOI-ID10.14670/HH-18-258
dc.identifier.issn02133911
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14160
dc.language.isoEnglish
dc.publisherHistology and Histopathology
dc.subjectAnimals
dc.subjectEstrous Cycle
dc.subjectExtracellular Signal-Regulated MAP Kinases
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectInsulin-Like Growth Factor I
dc.subjectMechanistic Target of Rapamycin Complex 1
dc.subjectOvary
dc.subjectPhosphatidylinositol 3-Kinase
dc.subjectPhosphorylation
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectRats, Wistar
dc.subjectSignal Transduction
dc.subjectTOR Serine-Threonine Kinases
dc.subjectUterus
dc.subjectinsulin-like growth factor-1, rat
dc.subjectmammalian target of rapamycin complex 1
dc.subjectmitogen activated protein kinase
dc.subjectmTOR protein, rat
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein kinase B
dc.subjectsomatomedin C
dc.subjecttarget of rapamycin kinase
dc.subjectanimal
dc.subjectcomparative study
dc.subjectenzymology
dc.subjectestrus cycle
dc.subjectfemale
dc.subjectimmunohistochemistry
dc.subjectmetabolism
dc.subjectovary
dc.subjectphosphorylation
dc.subjectsignal transduction
dc.subjectuterus
dc.subjectWistar rat
dc.titleImmunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages
dc.typeArticle

Files

Collections

Scopus Koleksiyonu

We collect and process your personal information for the following purposes: Authentication, Preferences, Acknowledgement and Statistics.
To learn more, please read our privacy policy.

Customize