Neurotoxic, cytotoxic, apoptotic and antiproliferative effects of some marine algae extracts on the NA2B cell line

Simple item page
dc.contributor.authorKurt O.
dc.contributor.authorÖzdal-Kurt F.
dc.contributor.authorAkçora C.M.
dc.contributor.authorÖzkut M.
dc.contributor.authorTuğlu M.I.
dc.date.accessioned2024-07-22T08:10:03Z
dc.date.available2024-07-22T08:10:03Z
dc.date.issued2018
dc.description.abstractOxidative stress contributes to cancer pathologies and to apoptosis. Marine algae exhibit cytotoxic, antiproliferative and apoptotic effects; their metabolites have been used to treat many types of cancer. We investigated in culture extracts of Petalonia fascia, Jania longifurca and Halimeda tuna to determine their effects on mouse neuroblastoma cell line, NA2B. NA2B cells were treated with algae extracts, and the survival and proliferation of NA2B cells were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of algae extracts on oxidative stress in NA2B cells also were investigated using nitric oxide synthase (NOS) immunocytochemistry and apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling. We observed significant neurite inhibition with moderate damage by the neurotoxicity-screening test (NST) at IC50 dilutions of the extracts. MTT demonstrated that J. longifurca extracts were more toxic than P. fascia and H. tuna extracts. We found an increase of endothelial and inducible NOS immunostaining for oxidative stress and TUNEL analysis revealed increased apoptosis after application of extract. Our findings suggest that the algae we tested may have potential use for treatment of cancer. © 2018 The Biological Stain Commission.
dc.identifier.DOI-ID10.1080/10520295.2017.1381992
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15068
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCell Extracts
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectImmunohistochemistry
dc.subjectInhibitory Concentration 50
dc.subjectMarine Biology
dc.subjectMice
dc.subjectNeurites
dc.subjectPhaeophyta
dc.subjectcell extract
dc.subjectanimal
dc.subjectapoptosis
dc.subjectbrown alga
dc.subjectcell proliferation
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectIC50
dc.subjectimmunohistochemistry
dc.subjectmarine biology
dc.subjectmouse
dc.subjectneurite
dc.subjecttumor cell line
dc.titleNeurotoxic, cytotoxic, apoptotic and antiproliferative effects of some marine algae extracts on the NA2B cell line
dc.typeArticle

Files

Collections

Scopus Koleksiyonu