Role of the PON polymorphisms on progression of chronic hepatitis and cirrhosis; [Kronik hepatit ve siroz gelişiminde PON polimorfizmlerinin rolü]
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2011
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Aim and Background: The probable role of PON1-192, PON1-55, PON2-148, PON2-311 polymorphisms of the PON enzyme family, which are play a role in antioxidant pathways in the progression of chronic hepatitis to cirrhosis was investigated. Methods: The patient population included 64 chronic hepatitis patients without cirrhosis and 30 patients with cirrhosis which were diagnosed by biopsy compared to a control group (n=68) of normal healthy volunteers. All patients were recruited from the outpatient clinic of Gastroenterology, Celal Bayar University Faculty of Medicine. Genomic DNA of leukocytes was isolated by using a commercial isolation kit. PCR analysis was assessed for PON1-192, PON1-55, PON2-148, PON2-311 genotypes and the products digested with HinfI restriction enzyme to see the allelic polymorphisms. The PCR products were resolved on electrophoresis 2% agarose gel and visualized with a Syngene (USA) image analysis system. Odds ratios in 95% confidence interval were calculated for the two genotypes. For the statistical analyses SPSS version 10.0 was used. Results: The prevalence of PON1-192, PON1-55, PON2-148 genotypes were very different between the groups but was not statistically significant. The frequency of the "SS" and "SC" genotypes of PON2-311 in patients with hepatitis and cirrhosis was higher than the control group and their Odds Ratios were statistically significant. Conclusion: The prevalence of PON2 gene "SS" and "SC" genotypes of in patients was higher than that of healthy volunteers (ORs were 3,855 and 2,404 respectively). Similarly "SS" and "SC" genotypes of in patients with cirrhosis was higher than that of patients with hepatitis (ORs were 3,436 and 2,223 respectively) These results suggested that the "SS" and "SC" genotypes of PON2 gene might cause a susceptibility for developing of hepatitis and progression of this condition to cirrhosis. It can be also speculated that "CC" genotype may be protective for the progression of disease. © TurkJBiochem.com.
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aryldialkylphosphatase , enzyme , genomic DNA , agar gel electrophoresis , antioxidant activity , article , chronic hepatitis , chronic liver disease , confidence interval , controlled study , disease course , DNA sequence , enzyme activity , genetic polymorphism , genotype , human , human cell , image analysis , imaging system , leukocyte , liver , liver cirrhosis , liver disease , major clinical study , pathogenesis , polymerase chain reaction , prevalence , statistical analysis