Evaluation of serological tests for diagnosis of brucellosis

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dc.contributor.authorPabuccuoglu O.
dc.contributor.authorEcemis T.
dc.contributor.authorEl S.
dc.contributor.authorCoskun A.
dc.contributor.authorAkcali S.
dc.contributor.authorSanlidag T.
dc.date.accessioned2024-07-22T08:20:11Z
dc.date.available2024-07-22T08:20:11Z
dc.date.issued2011
dc.description.abstractThe aim of the present study was to compare serological tests (Rose Bengal [RB]; standard agglutination test [SAT]; enzyme immunoassay [EIA] for detection of IgM, IgA, and IgG; and 2- mercaptoethanol [2-ME] test) that are routinely used in patients prediagnosed with different clinical types of brucellosis (acute, subacute, or chronic), and to evaluate the results of the IgG avidity test. Ninety-two patients having titers>1/160 as measured by SAT were included in the study. The IgG avidity test was performed in 78 patients who had positive EIA-IgG results. RB test results were positive in 88 (95.7z) patients. A statistically significant correlation was found between a positive EIA-IgM result and the diagnosis of acute brucellosis. When compared to the results of the SAT, the 2-ME test showed a lower titer in 55 (59.8z) patients, and the agreement between the 2-ME test and EIA-IgG was calculated as 84.8z. No statistical difference was found between the 40z avidity index used in the IgG avidity test and avidity maturation time (6 months). From our study, we concluded that (i) the RB and SAT tests are appropriate and reliable tests for the serological diagnosis of brucellosis; (ii) IgM can be used as a marker of acute brucellosis; (iii) the 2-ME test, similar to EIA, can be used to determine IgM levels; and (iv) the IgG avidity test should be standardized.
dc.identifier.issn18842836
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18039
dc.language.isoEnglish
dc.subjectAdult
dc.subjectAged
dc.subjectAgglutination Tests
dc.subjectAntibodies, Bacterial
dc.subjectBrucella
dc.subjectBrucellosis
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunoenzyme Techniques
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectMale
dc.subjectMercaptoethanol
dc.subjectMiddle Aged
dc.subjectRose Bengal
dc.subjectSensitivity and Specificity
dc.subjectYoung Adult
dc.subjectimmunoglobulin A
dc.subjectimmunoglobulin G
dc.subjectimmunoglobulin M
dc.subjectmercaptoethanol
dc.subjectrose bengal
dc.subject2 mercaptoethanol test
dc.subjectacute disease
dc.subjectadult
dc.subjectaged
dc.subjectagglutination test
dc.subjectantibody detection
dc.subjectantibody titer
dc.subjectarticle
dc.subjectbrucellosis
dc.subjectchronic disease
dc.subjectenzyme immunoassay
dc.subjectevaluation
dc.subjectfemale
dc.subjecthuman
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectreliability
dc.subjectrose bengal test
dc.subjectserology
dc.titleEvaluation of serological tests for diagnosis of brucellosis
dc.typeArticle

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