Obtaining stem cell spheroids from foreskin tissue and the effect of corchorus olitorius l. On spheroid proliferation; [Sünnet derisinden kök hücre sferoidlerinin elde edilmesi ve corchorus olitorius l.’nin sferoid proliferasyonuna etkisi]

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dc.contributor.authorBecer E.
dc.contributor.authorSoykut G.
dc.contributor.authorKabadayi H.
dc.contributor.authorMammadov E.
dc.contributor.authorÇaliş İ.
dc.contributor.authorVatansever S.
dc.date.accessioned2024-07-22T08:08:07Z
dc.date.available2024-07-22T08:08:07Z
dc.date.issued2020
dc.description.abstractObjectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells. © Turk J Pharm Sci, Published by Galenos Publishing House.
dc.identifier.DOI-ID10.4274/tjps.galenos.2019.05658
dc.identifier.issn1304530X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14268
dc.language.isoEnglish
dc.publisherTurkish Pharmacists Association
dc.rightsAll Open Access; Gold Open Access
dc.subjectcaspase 3
dc.subjectCD34 antibody
dc.subjectflavanol derivative
dc.subjecthydrocinnamic acid
dc.subjectKi 67 antigen
dc.subjectreceptor type tyrosine protein phosphatase C
dc.subjectArticle
dc.subjectcell culture
dc.subjectcell growth
dc.subjectcell isolation
dc.subjectcell proliferation
dc.subjectcell structure
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectCorchorus olitorius
dc.subjectcytotoxicity
dc.subjecthigh performance liquid chromatography
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunofluorescence
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectliquid chromatography-mass spectrometry
dc.subjectmale
dc.subjectplant leaf
dc.subjectprepuce
dc.subjectspheroid cell
dc.subjecttissue repair
dc.titleObtaining stem cell spheroids from foreskin tissue and the effect of corchorus olitorius l. On spheroid proliferation; [Sünnet derisinden kök hücre sferoidlerinin elde edilmesi ve corchorus olitorius l.’nin sferoid proliferasyonuna etkisi]
dc.typeArticle

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