A new colorimetric method for rapid detection of ethambutol and streptomycin resistance in Mycobacterium tuberculosis: crystal violet decolorization assay (CVDA)

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dc.contributor.authorCoban A.Y.
dc.contributor.authorAkbal A.U.
dc.contributor.authorCeyhan I.
dc.contributor.authorUzun M.
dc.contributor.authorSertel Selale D.
dc.contributor.authorAslan G.
dc.contributor.authorDelialioglu N.
dc.contributor.authorOzyurt M.
dc.contributor.authorBektore B.
dc.contributor.authorBicmen C.
dc.contributor.authorAslanturk A.
dc.contributor.authorUcarman N.
dc.contributor.authorAlbay A.
dc.contributor.authorSig A.K.
dc.contributor.authorOzkutuk N.
dc.contributor.authorSurucuoglu S.
dc.date.accessioned2024-07-22T08:08:42Z
dc.date.available2024-07-22T08:08:42Z
dc.date.issued2019
dc.description.abstractStreptomycin (STR) and ethambutol (EMB) are important drugs used for the treatment of tuberculosis. There is a need for fast, reliable and inexpensive methods for detecting resistance to these drugs. The aim of this study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for the detection of STR and EMB resistance that is important drugs in tuberculosis treatment. In this study, drug susceptibility testing was performed on 140 Mycobacterium tuberculosis isolates provided from nine centers. Three tubes were used for each isolate. One of the tubes had a concentration of 2 mg/L STR and the other 5 mg/L EMB. The third was drug-free control tube. Sensitivity, specificity, positive predictive value (PPD), negative predictive value (NPD) and agreement for STR were found to be 81.8%, 94.6%, 87.8%, 91.5% and 90.57%, respectively. For EMB, sensitivity, specificity, PPD, NPD, and agreement were found to be 76%, 98.23%, 90.47%, 94.87% and 94.2%, respectively. The results were obtained in 11.3 ± 2.7 days (8–21 days). CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of STR and EMB resistance, and it could be adapted for drug susceptibility testing. © 2018, Springer Nature Switzerland AG.
dc.identifier.DOI-ID10.1007/s10482-018-1198-2
dc.identifier.issn00036072
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14491
dc.language.isoEnglish
dc.publisherSpringer Netherlands
dc.subjectAnti-Bacterial Agents
dc.subjectBacterial Typing Techniques
dc.subjectColorimetry
dc.subjectDrug Resistance, Bacterial
dc.subjectEthambutol
dc.subjectGentian Violet
dc.subjectHumans
dc.subjectMycobacterium tuberculosis
dc.subjectStreptomycin
dc.subjectTuberculosis
dc.subjectcrystal violet
dc.subjectethambutol
dc.subjectstreptomycin
dc.subjectantiinfective agent
dc.subjectethambutol
dc.subjectstreptomycin
dc.subjectantibiotic resistance
dc.subjectantibiotic sensitivity
dc.subjectArticle
dc.subjectcolorimetry
dc.subjectconcentration (parameter)
dc.subjectcontrolled study
dc.subjectcrystal violet decolorization assay
dc.subjectdecolorization
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectpredictive value
dc.subjectpriority journal
dc.subjectsensitivity and specificity
dc.subjectantibiotic resistance
dc.subjectbacterium identification
dc.subjectchemistry
dc.subjectcolorimetry
dc.subjectdrug effect
dc.subjectevaluation study
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectMycobacterium tuberculosis
dc.subjectprocedures
dc.subjecttuberculosis
dc.titleA new colorimetric method for rapid detection of ethambutol and streptomycin resistance in Mycobacterium tuberculosis: crystal violet decolorization assay (CVDA)
dc.typeArticle

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