Temperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples; [Serum Örneklerinde Anti-Toxoplasma gondii Antikor Titrelerinin Sıcaklık ve Zamana Bağlı Değişimi]

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dc.contributor.authorÇoban E.
dc.contributor.authorKilimcioğlu A.A.
dc.date.accessioned2024-07-22T08:06:12Z
dc.date.available2024-07-22T08:06:12Z
dc.date.issued2021
dc.description.abstractObjective: This study aimed to determine the temperature-and time-dependent variations in the anti-Toxoplasma gondii (T. gondii) antibody titres in serum samples collected from T. gondii-infected patients. Methods: During May 2017-February 2018, five serum samples (stored at different time periods and conditions) from pregnant or non-pregnant women aged ≥18 years who were infected with T. gondii and had applied to our Parasitology Department of Hafsa Sultan Hospital, Manisa Celal Bayar University, were investigated for the anti-T. gondii IgG antibody levels by enzyme linked fluorescent assay (ELFA). Results: The serum samples of five female volunteers who were infected with T. gondii that were stored at room temperature (20/25 °C), in a cargo package (+4/+8 °C), in a refrigerator (+4 °C), in a deep freezer (-16/-20 °C) and in an incubator (+37 °C) were tested at 0, 24, 48 and 72 hours after infection with the ELFA test. No statistically significant difference was observed in the anti-T. gondii IgG antibody titres (p>0.05). Conclusion: The results obtained from the patients infected with T. gondii at different times and conditions of up to 72 hours were not significantly affected clinically. Hence, more comprehensive data can be obtained by increasing the number of patients and storing the serum samples for more than 72 hours. © 2021, Galenos Publishing House. All rights reserved.
dc.identifier.DOI-ID10.4274/tpd.galenos.2020.7074
dc.identifier.issn13006320
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13428
dc.language.isoTurkish
dc.publisherGalenos Publishing House
dc.rightsAll Open Access; Gold Open Access
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAntibodies, Protozoan
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectMiddle Aged
dc.subjectSpecimen Handling
dc.subjectTemperature
dc.subjectTime Factors
dc.subjectToxoplasma
dc.subjectToxoplasmosis
dc.subjectprotozoon antibody
dc.subjectadolescent
dc.subjectadult
dc.subjectblood
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthuman
dc.subjectimmunology
dc.subjectisolation and purification
dc.subjectmiddle aged
dc.subjectspecimen handling
dc.subjecttemperature
dc.subjecttime factor
dc.subjectToxoplasma
dc.subjecttoxoplasmosis
dc.titleTemperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples; [Serum Örneklerinde Anti-Toxoplasma gondii Antikor Titrelerinin Sıcaklık ve Zamana Bağlı Değişimi]
dc.typeArticle

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