An experimental model of hemolysis-induced acute pancreatitis

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dc.contributor.authorSaruc M.
dc.contributor.authorYuceyar H.
dc.contributor.authorTurkel N.
dc.contributor.authorOzutemiz O.
dc.contributor.authorTuzcuoglu I.
dc.contributor.authorYuce G.
dc.contributor.authorHuseyinov A.
dc.date.accessioned2024-07-22T08:24:47Z
dc.date.available2024-07-22T08:24:47Z
dc.date.issued2003
dc.description.abstractThe literature indicates that acute pancreatitis is a complication of massive hemolysis with a prevalence of about 20%. We describe an experimental model of hemolysis-induced acute pancreatitis. Hemolytic anemia was induced in rats by a single ip injection of 60 mg/kg of 20 mg/ml acetylphenylhydrazine (APH) in 20% (v/v) ethanol on the first experimental day (day 0). One hundred and fifty Wistar albino rats weighing 180-200 g were divided into three groups of 50 animals each: groups 1, 2 and 3 were injected ip with APH, 20% ethanol, and physiological saline, respectively. Ten rats from each group were sacrificed on study days 1, 2, 3, 4 and 5. Serum amylase, lipase levels and pancreatic tissue tumor necrosis factor-α (TNF-α) and platelet-activating factor (PAF) contents were determined and a histological examination of the pancreas was performed. No hemolysis or pancreatitis was observed in any of the rats in groups 2 and 3. In group 1, massive hemolysis was observed in 35 (70%) of 50 rats, moderate hemolysis in seven (14%), and no hemolysis in eight (16%). Thirty-three of 35 (94.2%) rats with massive hemolysis had hyperamylasemia, and 29 of these rats (82.8%) had histologically proven pancreatitis. The most severe pancreatitis occurred on day 3, as demonstrated by histology. Tissue TNF-α and PAF levels were statistically higher in group 1 than in groups 2 and 3. Acute massive hemolysis induced acute pancreatitis, as indicated by histology, in almost 80% of cases. Hemolysis may induce acute pancreatitis by triggering the release of proinflammatory and immunoregulatory cytokines.
dc.identifier.DOI-ID10.1590/S0100-879X2003000700008
dc.identifier.issn0100879X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20127
dc.language.isoEnglish
dc.publisherAssociacao Brasileira de Divulgacao Cientifica
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAnimalia
dc.subjectacetylphenylhydrazine
dc.subjectalcohol
dc.subjectamylase
dc.subjectcytokine
dc.subjectthrombocyte activating factor
dc.subjecttriacylglycerol lipase
dc.subjecttumor necrosis factor alpha
dc.subjectacute pancreatitis
dc.subjectamylase blood level
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectcytokine release
dc.subjectdisease severity
dc.subjectexperimental model
dc.subjecthemolysis
dc.subjecthemolytic anemia
dc.subjecthistopathology
dc.subjectnonhuman
dc.subjectrat
dc.subjectrat strain
dc.subjecttissue level
dc.subjecttriacylglycerol lipase blood level
dc.titleAn experimental model of hemolysis-induced acute pancreatitis
dc.typeArticle

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