The neuroprotective effect of pioglitazone on NB2a mouse neuroblastoma cell culture; [Pioglitazonun NB2a fare nöroblastoma hücre kültürüdeki nöroprotektif etkisi]

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dc.contributor.authorVural K.
dc.contributor.authorSeyrek O.
dc.date.accessioned2024-07-22T08:09:17Z
dc.date.available2024-07-22T08:09:17Z
dc.date.issued2019
dc.description.abstractPioglitazone (PGT) is a PPAR-γ activator that has neuroprotective properties via different mechanisms. It is thought to be neuroprotective in both acute and chronic use. Chlorpyrifos (CPS) is an Organophosphate insecticide that leads to attention deficit and cognitive problems in children and its neurotoxic effects are well known. This study aims to investigate the neuroprotective effects of PGT on CPS neurotoxicity in NB2a cell in the culture medium. We investigated the cell viability and proliferation using MTT assay and the percentage of neurite inhibition was analysed by measuring neurite outgrowth. Apoptosis was evaluated using the apoptotic index in TUNEL staining. Cell proliferation was found to be significantly reduced by CPS (25 µM), and this concentration-based reduction was prevented by PGT. Neurite outgrowth was inhibited by CPS (25 µM), whereas PGT significantly reversed neurite inhibition at and above 10 μM concentrations. The apoptotic index, which was increased using CPS (25 µM), was observed to reduce using PGT, depending on the concentration. Organophosphate is harmful to human health, and to our knowledge, there is no treatment. In individuals exposed to chlorpyrifos toxicity, acute toxic effects on neurons may be prevented or treated by PGT. © 2019, Veteriner Fakultesi Dergisi. All rights reserved.
dc.identifier.DOI-ID10.9775/kvfd.2018.19940
dc.identifier.issn13006045
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14765
dc.language.isoEnglish
dc.publisherVeteriner Fakultesi Dergisi
dc.rightsAll Open Access; Gold Open Access
dc.subjectpioglitazone
dc.subjectanimal cell
dc.subjectapoptosis
dc.subjectArticle
dc.subjectattention deficit disorder
dc.subjectcell culture
dc.subjectcell density
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcognitive defect
dc.subjectcontrolled study
dc.subjectdegenerative disease
dc.subjectimmunohistochemical score
dc.subjectmouse
dc.subjectMTT assay
dc.subjectneurite outgrowth
dc.subjectneuroprotection
dc.subjectneurotoxicity
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectscoring system
dc.subjectTUNEL assay
dc.titleThe neuroprotective effect of pioglitazone on NB2a mouse neuroblastoma cell culture; [Pioglitazonun NB2a fare nöroblastoma hücre kültürüdeki nöroprotektif etkisi]
dc.typeArticle

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