Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

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dc.contributor.authorWang, L
dc.contributor.authorKaya, HB
dc.contributor.authorZhang, N
dc.contributor.authorRai, R
dc.contributor.authorWillmann, MR
dc.contributor.authorCarpenter, SCD
dc.contributor.authorRead, AC
dc.contributor.authorMartin, F
dc.contributor.authorFei, ZJ
dc.contributor.authorLeach, JE
dc.contributor.authorMartin, GB
dc.contributor.authorBogdanove, AJ
dc.date.accessioned2025-04-10T10:36:28Z
dc.date.available2025-04-10T10:36:28Z
dc.description.abstractPrime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in Nicotiana benthamiana, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.
dc.identifier.e-issn2673-3439
dc.identifier.urihttp://hdl.handle.net/20.500.14701/42335
dc.language.isoEnglish
dc.titleSpelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
dc.typeArticle

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