Is administration of n-3 fatty acids by mucosal enema protective against trinitrobenzene-induced colitis in rats?

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dc.contributor.authorYuceyar H.
dc.contributor.authorOzutemiz O.
dc.contributor.authorHuseyinov A.
dc.contributor.authorSaruç M.
dc.contributor.authorAlkanat M.
dc.contributor.authorBor S.
dc.contributor.authorCoker I.
dc.contributor.authorBatur Y.
dc.date.accessioned2024-07-22T08:25:49Z
dc.date.available2024-07-22T08:25:49Z
dc.date.issued1999
dc.description.abstractWe investigated the protective role of fish oil (FO-source of n-3 FA) enriched diet (in the first protocol) in 20 rats and FO administration intrarectally (in the second protocol) in 40 rats with trinitrobennzene (TNB) colitis. All colonic specimens were pathologically evaluated, myeloperoxidase enzyme activities were measured, leukotriene B4 (LTB4) and LTC4 levels were determined by radioimmunoassay. In the first protocol 10 rats (group A1) were fed with 8% sunflower and cotton oil enriched diet and (group A2) with 8% FO enriched diet for 6 weeks. At the end of this period, TNB (30 mg in 0.25 ml of 30% ethanol) were intrarectally administered. After 2 weeks, rats were sacrificed. MPO activities (2.47 versus 30.17), LTB4 (34.5 versus 903.3) and LTC4 (77.7 versus 456.0) levels were significantly reduced in group A2 compared with group A1 (P<0.005). There was also a significant difference in pathologic scores (1.55 versus 2.12, P<0.002) between two groups. In the first part of the second protocol, 20 male rats were randomized into two equal groups (B1 and B2) and TNB colitis was induced. After 1 day, 1 ml of saline (group B1) or n-3 FA enemas (group B2) were administered every day for 2 weeks. At the end of this period, rats were sacrificed and evaluated as done for previous groups. Although there was no significant difference between the two groups in comparison with MPO enzyme activities and pathologic scores, the LTB4 (130.1 versus 971.0) and LTC4 (126.0 versus 532.0) levels of FO group were significantly reduced (P<0.005). In the second part of the second protocol, 20 male rats were randomized into two groups. One millilitre of saline (group B3) or FO enemas (group B4) were administered to rats every day for 3 days. At the fourth day, TNB-colitis was induced and after 24 h rats were sacrificed. We could not find any significant difference in MPO activities, pathologic scores, LTB4 and LTC4 levels between groups B3 and B4. In conclusion; FO enriched diet decreased both pathologic damage and tissue LT levels. The second protocol of our study revealed that the long-term FO enemas decreased the LTB4 and LTC4 levels; however, did not have any beneficial effect on the tissue lesions. Short periods of FO enemas did not have a protective role in the occurrence of experimental colitis. The present study showed that FO enemas significantly decreased LT levels. The protective effect of FO (oral and enema) in TNB colitis may open a new insight into the treatment of inflammatory bowel disease. (C) 1999 Harcourt Publishers Ltd.
dc.identifier.DOI-ID10.1054/plef.1999.0111
dc.identifier.issn09523278
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20613
dc.language.isoEnglish
dc.publisherChurchill Livingstone
dc.subjectalcohol
dc.subjectenema
dc.subjectfish oil
dc.subjectleukotriene
dc.subjectleukotriene B4
dc.subjectleukotriene C4
dc.subjectmyeloperoxidase
dc.subjectnitrobenzene derivative
dc.subjectomega 3 fatty acid
dc.subjectsunflower oil
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectarticle
dc.subjectcolitis
dc.subjectcontrolled study
dc.subjectdiet
dc.subjectenzyme activity
dc.subjecthistopathology
dc.subjectmale
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprostaglandin release
dc.subjectradioimmunoassay
dc.subjectrat
dc.subjectstomach protection
dc.titleIs administration of n-3 fatty acids by mucosal enema protective against trinitrobenzene-induced colitis in rats?
dc.typeArticle

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