Role of intercellular communications in breast cancer multicellular tumor spheroids after chemotherapy

dc.contributor.authorOktem G.
dc.contributor.authorBilir A.
dc.contributor.authorAyla S.
dc.contributor.authorYavasoglu A.
dc.contributor.authorGoksel G.
dc.contributor.authorSaydam G.
dc.contributor.authorUysal A.
dc.date.accessioned2024-07-22T08:23:34Z
dc.date.available2024-07-22T08:23:34Z
dc.date.issued2006
dc.description.abstractTumor heterogeneity is an important feature that is especially involved in tumor aggressiveness. Multicellular tumor spheroids (MTS) may provide some benefits in different steps for investigation of the aggregation, organization, differentiation, and network formation of tumor cells in 3D space. This model offers a unique opportunity for improvements in the capability of a current strategy to detect the effect of an appropriate anticancer agent. The aim of this study was to investigate the cellular interactions and morphological changes following chemotherapy in a 3D breast cancer spheroid model. Distribution of the gap junction protein "connexin-43" and the tight junction protein "occludin" was investigated by immunohistochemistry. Cellular interactions were examined by using transmission and scanning electron microscopies as well as light microscopy with Giemsa staining after treating cells with doxorubicin, docetaxel, and doxorubicin/docetaxel combination. Statistical analyses showed significant changes and various alterations that were observed in all groups; however, the most prominent effect was detected in the doxorubicin/docetaxel combination group. Distinct composition as a vessel-like structure and a pseudoglandular pattern of control spheroids were detected in drug-administered groups. Immunohistochemical results were consistent with the ultrastructural changes. In conclusion, doxorubicin/ docetaxel combination may be more effective than the single drug usage as shown in a 3D model. The MTS model has been found to be an appropriate and reliable method for the detection of the changes in the expression of cellular junction proteins as well as other cellular proteins occurring after chemotherapy. The MTS model can be used to validate the effects of various combinations or new chemotherapeutic agents as well as documentation of possible mechanisms of new drugs. Copyright © 2006 Cognizant Comm. Corp.
dc.identifier.DOI-ID10.3727/000000006783981071
dc.identifier.issn09650407
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19574
dc.language.isoEnglish
dc.publisherTech Science Press
dc.subjectconnexin 43
dc.subjectdocetaxel
dc.subjectdoxorubicin
dc.subjectoccludin
dc.subjectarticle
dc.subjectbreast cancer
dc.subjectcell communication
dc.subjectcell interaction
dc.subjectcell structure
dc.subjectcellular distribution
dc.subjectcombination chemotherapy
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectdrug effect
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC 50
dc.subjectimmunohistochemistry
dc.subjectmonotherapy
dc.subjectmulticellular spheroid
dc.subjectpriority journal
dc.subjectprotein localization
dc.subjectscanning electron microscopy
dc.subjecttransmission electron microscopy
dc.titleRole of intercellular communications in breast cancer multicellular tumor spheroids after chemotherapy
dc.typeArticle

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