P53 intronic variant G13964C analyses in cases with colon cancer

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dc.contributor.authorOnrat S.T.
dc.contributor.authorEllidokuz E.
dc.contributor.authorKüpelioǧlu A.
dc.contributor.authorDurhan E.
dc.date.accessioned2024-07-22T08:21:31Z
dc.date.available2024-07-22T08:21:31Z
dc.date.issued2009
dc.description.abstractNucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. G13964C base change functioned as dominant mutation similar to the more common missense, nonsense and splice-site mutations. To detect the G13964C variant PCR-RFLP assay was used. In this study, DNA was isolated from colon cancer tissue samples of 35 cases (19 female and 16 male) diagnosed to be colon carcinoma. In this study, we found that mutations were present in 30 (85.7%) of 35 cases enrolled into study. In 7 (23.3%) cases G/G, 21 (70.0%) cases G/C and 2 (6.7%) C/C genotypes were found. In 5 (14.3%) cases DNA isolation could not be obtained. Our results indicate that heterozygotes for the GC allele have higher frequency than other alleles and one of the reasons of colon cancer may be related to GC allele frequency.
dc.identifier.issn10193103
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18660
dc.language.isoEnglish
dc.subjectprotein p53
dc.subjectadult
dc.subjectaged
dc.subjectarticle
dc.subjectcancer grading
dc.subjectclinical article
dc.subjectcolon cancer
dc.subjectcontrolled study
dc.subjectDNA isolation
dc.subjectfemale
dc.subjectgene frequency
dc.subjectgene mutation
dc.subjectgene overexpression
dc.subjectgenetic analysis
dc.subjectgenetic association
dc.subjectgenetic polymorphism
dc.subjectgenetic variability
dc.subjectgenotype
dc.subjectheterozygosity
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectintron
dc.subjectmale
dc.subjectnucleotide sequence
dc.subjectrestriction fragment length polymorphism
dc.titleP53 intronic variant G13964C analyses in cases with colon cancer
dc.typeArticle

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