Simultaneous folate intake may prevent advers effect of valproic acid on neurulating nervous system

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dc.contributor.authorUmur A.S.
dc.contributor.authorSelcuki M.
dc.contributor.authorBursali A.
dc.contributor.authorUmur N.
dc.contributor.authorKara B.
dc.contributor.authorVatansever H.S.
dc.contributor.authorDuransoy Y.K.
dc.date.accessioned2024-07-22T08:19:29Z
dc.date.available2024-07-22T08:19:29Z
dc.date.issued2012
dc.description.abstractPurpose: The aim of this study is to elucidate the preventive effect of folic acid (FA) on teratogenic effects of valporic acid (VA) in early stage chick embryos on neural tube development. Materials and methods: One hundred and fifty specific pathogen-free (SPF) chick eggs were used to investigate the neurulation in five groups. Group A was the control group. Group B was injected 0.02 ml of saline (0.9% NaCl) and was used for sham group. VA (0.72 mg) in 0.02 ml saline was injected in Group C, and 0.342 mcg of FA in 0.02 ml NaCl were administered to the embryos in Group D. VA (0.72 mg)+0.342 mcg of FA in 0.02 ml saline were administered simultaneously to the eggs in Group E. At the end of 72 h, all embryos were extracted from eggs and were fixed, and for histological analyses hematoxylin and eosine was used, for detection of apoptotic cells terminal deoxyribonucleotide transferase-mediated dUTP-X nick end labeling (TUNEL) was used and for distribution of P53, bcl-2 and caspase-3, caspase-6, caspase-8 and caspase-9 immunoperoxidase techniques were used. Results: While there were no neural tube defects in the embryos of groups A, B and D, eight embryos died in group C and there were 12 embryos with retarded embryological development. In contrast to that, no death was observed in group E, but only eight embryos were detected with maldevelopmental delay stage. Conclusion: These results suggested that VA may induce apoptotic mechanisms but not through the p53 pathway. In addition, FA effectively prevents the teratogenic influence of VA on chick embryo at neurulation stages by stopping cascade of apoptosis before caspase 3 expression. © 2012 Springer-Verlag.
dc.identifier.DOI-ID10.1007/s00381-011-1673-9
dc.identifier.issn14330350
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17691
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectAnticonvulsants
dc.subjectChick Embryo
dc.subjectEmbryonic Development
dc.subjectFolic Acid
dc.subjectNervous System
dc.subjectNeural Tube Defects
dc.subjectNeurulation
dc.subjectValproic Acid
dc.subjectcaspase 3
dc.subjectcaspase 6
dc.subjectcaspase 8
dc.subjectcaspase 9
dc.subjectfolic acid
dc.subjectprotein bcl 2
dc.subjectprotein p53
dc.subjectvalproic acid
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectchick embryo
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectembryo
dc.subjecthistology
dc.subjectimmunohistochemistry
dc.subjectimmunoperoxidase staining
dc.subjectneural tube
dc.subjectneurulation
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprophylaxis
dc.subjectprotein localization
dc.subjectteratogenicity
dc.subjectvitamin intake
dc.titleSimultaneous folate intake may prevent advers effect of valproic acid on neurulating nervous system
dc.typeArticle

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