Evaluation of the relationship between inducible nitric oxide synthase (iNOS) activity and effects of melatonin in experimental osteoporosis in the rat

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dc.contributor.authorOktem G.
dc.contributor.authorUslu S.
dc.contributor.authorVatansever S.H.
dc.contributor.authorAktug H.
dc.contributor.authorYurtseven M.E.
dc.contributor.authorUysal A.
dc.date.accessioned2024-07-22T08:23:22Z
dc.date.available2024-07-22T08:23:22Z
dc.date.issued2006
dc.description.abstractInducible nitric oxide synthase (iNOS) plays a critical role in the pathogenesis of osteoporosis. iNOS generates nitric oxide (NO), a free radical contributing to the imbalance between bone formation and resorption caused by estrogen depletion. Melatonin is the major product of the pineal gland which is known to diminish iNOS expression and NO production significantly. The aim of this study was to determine the distribution of iNOS and the amount of apoptotic cells after melatonin treatment in ovariectomized rats. Since previous studies have shown that constitution of bone formation is primarily sustained in nucleus pulposus and epiphyseal cartilage, experiments were carried out on nucleus pulposus and epiphyseal cartilage; additional quantitation of osteoblasts and osteoclasts were evaluated on vertebral area as well. Vertebral sections of ovariectomized rats were obtained from formalin-fixed and parafin-embedded blocks. iNOS expression and quantitation of apoptotic cells in nucleus pulposus and epiphyseal cartilage were evaluated using indirect immunoperoxidase and TUNEL techniques, respectively. The number of osteoclasts and osteoblasts in trabecular bone was determined using histomorphometry. Ovariectomy increased iNOS expression and the number of apoptotic cells in nucleus pulposus and epiphyseal cartilage, whereas a 4-week treatment with melatonin (10 mg/kg/day) resulted in the reduction of both effects. These data indicate that there is strong influence of melatonin application on expression of iNOS, apoptosis, osteoclast and osteoblast numbers after ovariectomy. In conclusion, melatonin besides its usual use as an antiaging hormone, may also be an effective hormone in treatment of bone changes in estrogen deficiency states. © Springer-Verlag 2006.
dc.identifier.DOI-ID10.1007/s00276-005-0065-9
dc.identifier.issn09301038
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19495
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectAntioxidants
dc.subjectApoptosis
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectGrowth Plate
dc.subjectImmunoenzyme Techniques
dc.subjectIn Situ Nick-End Labeling
dc.subjectIntervertebral Disk
dc.subjectMelatonin
dc.subjectNitric Oxide Synthase Type II
dc.subjectOsteoblasts
dc.subjectOsteoclasts
dc.subjectOsteoporosis
dc.subjectOvariectomy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSodium Chloride
dc.subjectTime Factors
dc.subjectestrogen
dc.subjectfree radical
dc.subjectinducible nitric oxide synthase
dc.subjectmelatonin
dc.subjectnitric oxide
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectarticular cartilage
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectenzyme localization
dc.subjectepiphysis
dc.subjectestrogen deficiency
dc.subjectfemale
dc.subjectimmunoperoxidase staining
dc.subjectlumbar vertebra
dc.subjectmorphometrics
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectnucleus pulposus
dc.subjectossification
dc.subjectosteoblast
dc.subjectosteoclast
dc.subjectosteolysis
dc.subjectosteoporosis
dc.subjectovariectomy
dc.subjectpathogenesis
dc.subjectpineal body
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectrat
dc.subjecttrabecular bone
dc.titleEvaluation of the relationship between inducible nitric oxide synthase (iNOS) activity and effects of melatonin in experimental osteoporosis in the rat
dc.typeArticle

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