Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system

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dc.contributor.authorDeliloglu-Gurhan S.I.
dc.contributor.authorVatansever H.S.
dc.contributor.authorOzdal-Kurt F.
dc.contributor.authorTuglu I.
dc.date.accessioned2024-07-22T08:23:27Z
dc.date.available2024-07-22T08:23:27Z
dc.date.issued2006
dc.description.abstractBone marrow is a complex tissue composed of hematopoietic and stromal stem cells with the potential to differentiate into adipogenic, fibroblastic, reticular, osteogenic and chondrogenic lineages. Identification of differentiation markers during transformation of stromal cells into osteoblasts in a time-dependent manner may be informative for cell-based tissue engineering. Therefore, we investigated the effects of osteogenic medium (OM) on the proliferation and differentiation of rat bone marrow stromal cells (BMSCs). BMSCs from adult male rat tibia and femur were collected and cultured in α-MEM medium with 10% fetal bovine serum, penicillin, streptomycin and gentamycin. After three days of culture, the medium covering the adherent cells in culture was changed to OM containing dexamethasone, Na-β-glycerophosphate and ascorbic acid. As a control, cell culture was also continued in the original medium for the same time period. Differentiated osteoblast cells were collected after 7, 10, 14, 21 and 30 days of culture, fixed with 4% paraformaldehyde and their immunolabelling for osteoblast markers osteonectin (ON) and osteocalcin (OC) was assessed using an indirect immunoperoxidase technique. Immunoabelling of ON and OC was detectable from day 10 of culture, began to increase on day 14, and increased steadily through to day 21. Labelling was highest on day 30 and was more intense in cells cultured with OM compared to the culture without OM. The control cells cultured in the absence of OM produced negligible levels of both markers. In conclusion, our culture system facilitated differentiation of BMSCs into osteoblasts featuring osteoblast markers, and these cells may be useful in autologous bone implant for the treatment of bone wound healing. © 2005 Elsevier GmbH. All rights reserved.
dc.identifier.DOI-ID10.1016/j.acthis.2005.11.001
dc.identifier.issn00651281
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19544
dc.language.isoEnglish
dc.publisherElsevier GmbH
dc.subjectAnimalia
dc.subjectBovinae
dc.subjectBiomarkers
dc.subjectBone
dc.subjectCarboxylic acids
dc.subjectCytology
dc.subjectTissue culture
dc.subjectascorbic acid
dc.subjectbovine serum albumin
dc.subjectcell marker
dc.subjectdexamethasone
dc.subjectgentamicin
dc.subjectglycerol 2 phosphate
dc.subjectosteocalcin
dc.subjectosteonectin
dc.subjectparaformaldehyde
dc.subjectpenicillin G
dc.subjectperoxidase
dc.subjectstreptomycin
dc.subjectBone marrow stromal cell
dc.subjectOsteoblast
dc.subjectOsteocalcin
dc.subjectOsteonectin
dc.subjectTissue engineering
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectantibody labeling
dc.subjectarticle
dc.subjectbone development
dc.subjectbone marrow cell
dc.subjectcell adhesion
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcell structure
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectfemur
dc.subjectimmunocytochemistry
dc.subjectmale
dc.subjectnonhuman
dc.subjectosteoblast
dc.subjectrat
dc.subjectstroma cell
dc.subjecttibia
dc.subjecttissue characterization
dc.subjecttissue engineering
dc.subjectCells
dc.titleCharacterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system
dc.typeArticle

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