Investigation of the effects of rapamycin on the mTOR pathway and apoptosis in metastatic and non-metastatic human breast cancer cell lines

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dc.contributor.authorEkizceli G.
dc.contributor.authorUluer E.T.
dc.contributor.authorInan S.
dc.date.accessioned2024-07-22T08:08:06Z
dc.date.available2024-07-22T08:08:06Z
dc.date.issued2020
dc.description.abstractAIM: The aim of this study was to analyze the effects of rapamycin treatment on apoptosis via mTOR pathway in metastatic and non-metastatic human breast cancer cell lines by immunohistochemical and TUNEL analysis. METHOD: MCF-7 and MDA-MB 231 cell lines were incubated under standard conditions forming Rapamycin and control groups. In immunohistochemical evaluation; mTOR pathway was evaluated with anti-IGF1, anti-PI3K, anti-pAKT1/2/3, anti-mTORC1, anti-mTORC2 and anti-ERK1 antibodies. The effect of apoptosis was also confi rmed by TUNEL method. RESULTS: In this study, activation of PI3K/AKT/mTOR and related molecular pathways in the MDA-MB 231 and MCF-7 breast cancer cell line was evaluated and it was observed that these pathways could play a key role in cancer development. Increased apoptotic cells were observed in mTORC1 inhibition by Rapamycin administration. CONCLUSION: Targeting the mTOR pathway in breast cancer treatment may be a treatment option. In addition, the demonstration and confi rmation of increased apoptosis in Rapamycin treated groups suggested that Rapamycin, an inhibitor of mTOR, is promising in the treatment of breast cancer (Tab. 2, Fig. 3, Ref. 66). © 2020, Comenius University.
dc.identifier.DOI-ID10.4149/BLL_2020_049
dc.identifier.issn00069248
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14260
dc.language.isoEnglish
dc.publisherComenius University
dc.rightsAll Open Access; Bronze Open Access
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectHumans
dc.subjectMCF-7 Cells
dc.subjectNeoplasm Metastasis
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectSirolimus
dc.subjectTOR Serine-Threonine Kinases
dc.subjectMTOR protein, human
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein kinase B
dc.subjectrapamycin
dc.subjecttarget of rapamycin kinase
dc.subjectapoptosis
dc.subjectbreast tumor
dc.subjectcell proliferation
dc.subjecthuman
dc.subjectMCF-7 cell line
dc.subjectmetabolism
dc.subjectmetastasis
dc.subjectpathology
dc.subjecttumor cell line
dc.titleInvestigation of the effects of rapamycin on the mTOR pathway and apoptosis in metastatic and non-metastatic human breast cancer cell lines
dc.typeArticle

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