Analysis of artemisinin by a packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry technique

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dc.contributor.authorDost K.
dc.contributor.authorDavidson G.
dc.date.accessioned2024-07-22T08:24:50Z
dc.date.available2024-07-22T08:24:50Z
dc.date.issued2003
dc.description.abstractA packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry method was studied for the determination of artemisinin from Artemisia annua L. extracts. The technique does not require any kind of derivatisation prior to the analysis. All samples were simply dissolved in methanol and injected into the mobile phase. Detection was achieved by using mass spectrometry with atmospheric pressure chemical ionisation. The ionisation technique is relatively soft and provides protonated molecular ion and informative structural fragmentation for the compound. Benzophenone was used as a chromatographic standard for the determination of the analytical reproducibility. The supercritical carbon dioxide mobile phase used in the system was modified by 10% methanol. The average absolute retention time was 3.54 min with a standard deviation of 0.017 min and a relative standard deviation of 0.4% with respect to benzophenone for the procedure. The correlation coefficient was 0.998 and detection limit 370 pg on column.
dc.identifier.DOI-ID10.1039/b301342a
dc.identifier.issn00032654
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20153
dc.language.isoEnglish
dc.publisherRoyal Society of Chemistry
dc.subjectArtemisia annua extract
dc.subjectartemisinin
dc.subjectbenzophenone
dc.subjectcarbon dioxide
dc.subjectmethanol
dc.subjectArtemisia
dc.subjectarticle
dc.subjectderivatization
dc.subjectdrug structure
dc.subjectmass spectrometry
dc.subjectreproducibility
dc.subjectstructure analysis
dc.subjectsupercritical fluid chromatography
dc.titleAnalysis of artemisinin by a packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry technique
dc.typeArticle

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