The roles of transforming growth factor type β3(TGF-β3) and mast cells in the pathogenesis of scleroderma

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dc.contributor.authorKemal Ozbilgin M.
dc.contributor.authorInan S.
dc.date.accessioned2024-07-22T08:24:44Z
dc.date.available2024-07-22T08:24:44Z
dc.date.issued2003
dc.description.abstractScleroderma is a connective tissue disorder characterised by excessive accumulation of collagen in the skin and internal organs. The most likely explanation for this process is local activation of collagen synthesis from fibroblasts. Our intention was to elucidate whether TGF-β3 and mast cells play a pathogenic role in abnormal connective tissue formation in scleroderma. In this study, skin biopsies from 20 patients with scleroderma and five from healthy individuals were studied by an indirect immunoperoxidase technique to determine the immunoreactivity of TGF-β3 in the dermis. In addition, skin samples were stained with toluidine blue to count the number of mast cells in scleroderma, and tissues were examined under the electron microscope to evaluate the ultrastructural changes. Increased TGF-β3 immunoreactivities were detected in the dermis in the patient's skin, suggesting the presence of a subpopulation responsible for the increased collagen production. Mast cell counts in the skin of patients with scleroderma were significantly greater (19.2 plusmn; 4.1/unit) than those of normal controls (4.4 ± 1.2/unit). Ultrastructural observations indicated that there is a close relationship between the mast cells and fibroblasts. These results suggest that fibrosis in scleroderma could evolve through the activation of fibroblasts and the regulatory mechanisms that appear to modulate the behavior of these cells with respect to collagen production.
dc.identifier.DOI-ID10.1007/s10067-003-0706-5
dc.identifier.issn07703198
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20091
dc.language.isoEnglish
dc.subjectAdult
dc.subjectBiological Markers
dc.subjectBiopsy, Needle
dc.subjectCase-Control Studies
dc.subjectCell Movement
dc.subjectFemale
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectMale
dc.subjectMast Cells
dc.subjectMicroscopy, Electron
dc.subjectMiddle Aged
dc.subjectPhotomicrography
dc.subjectPrognosis
dc.subjectReference Values
dc.subjectScleroderma, Localized
dc.subjectSensitivity and Specificity
dc.subjectSkin
dc.subjectTransforming Growth Factor beta
dc.subjectTransforming Growth Factor beta3
dc.subjectcollagen
dc.subjecttolonium chloride
dc.subjecttransforming growth factor beta3
dc.subjectadult
dc.subjectarticle
dc.subjectcell subpopulation
dc.subjectcell ultrastructure
dc.subjectclinical article
dc.subjectcollagen synthesis
dc.subjectcontrolled study
dc.subjectdermis
dc.subjectelectron microscope
dc.subjectfemale
dc.subjectfibroblast
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunoperoxidase staining
dc.subjectimmunoreactivity
dc.subjectmale
dc.subjectmast cell
dc.subjectpathogenesis
dc.subjectpriority journal
dc.subjectregulatory mechanism
dc.subjectscleroderma
dc.subjectskin biopsy
dc.subjectskin fibrosis
dc.subjectstatistical significance
dc.subjecttransmission electron microscopy
dc.titleThe roles of transforming growth factor type β3(TGF-β3) and mast cells in the pathogenesis of scleroderma
dc.typeArticle

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