Serological screening of West Nile virus among blood donors in northern Cyprus

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dc.contributor.authorBalaman N.
dc.contributor.authorGazi U.
dc.contributor.authorImir T.
dc.contributor.authorSanlidag T.
dc.contributor.authorRuh E.
dc.contributor.authorTosun O.
dc.contributor.authorOzkul A.
dc.contributor.authorTaylan-Ozkan A.
dc.date.accessioned2024-07-22T08:07:26Z
dc.date.available2024-07-22T08:07:26Z
dc.date.issued2020
dc.description.abstractBackground: West Nile virus (WNV) is a neurotropic arbovirus that can also be transmitted through blood transfusion. Even though its geographic distribution has been expanding, there has not yet been any epidemiological data on WNV in northern Cyprus. The aim of our study is to fill this gap by using donated blood samples. Methods: Samples collected from the main government hospital blood bank in Nicosia were analyzed by anti-WNV enzyme-linked immunosorbent assay (ELISA) (immunoglobulin M [IgM] and immunoglobulin G [IgG]). Seropositive samples were subjected to plaque reduction neutralization test (PRNT) for confirmation and analyzed by ELISA IgG avidity test and reverse transcription real-time polymerase chain reaction (rRT-PCR). Results: Of the 760 sera samples, 2 (0.3%) were IgM+ and 31 (4.1%) were IgG+. Neutralization activity was detected in none (0.0%) of the IgM+ and 26 (83.9%) of IgG+ donor specimens. ELISA IgG avidity test reported high avidity in 21 (67.7%) and low avidity in one (3.2%) IgG+ sample. PRNT-confirmed anti-WNV IgG+ samples exhibited only borderline (19.2%) or high avidity (80.8%) values. rRT-PCR results were negative for both IgM+ and IgG+ samples. Conclusion: Anti-WNV antibodies were detected in northern Cyprus among blood donors. The establishment of preventive measures and evaluation of the geographic extent of the WNV in northern Cyprus are highly recommended. © 2020 Wiley Periodicals, Inc.
dc.identifier.DOI-ID10.1002/jmv.25669
dc.identifier.issn01466615
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13967
dc.language.isoEnglish
dc.publisherJohn Wiley and Sons Inc.
dc.subjectAntibodies, Viral
dc.subjectAntibody Affinity
dc.subjectBlood Donors
dc.subjectCyprus
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Viral
dc.subjectSeroepidemiologic Studies
dc.subjectWest Nile Fever
dc.subjectWest Nile virus
dc.subjectimmunoglobulin G antibody
dc.subjectimmunoglobulin M antibody
dc.subjectimmunoglobulin G
dc.subjectimmunoglobulin M
dc.subjectvirus antibody
dc.subjectvirus RNA
dc.subjectantibody detection
dc.subjectArticle
dc.subjectblood bank
dc.subjectblood donor
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectCyprus
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjectnonhuman
dc.subjectreal time reverse transcription polymerase chain reaction
dc.subjectscreening
dc.subjectvirus neutralization
dc.subjectvirus strain
dc.subjectWest Nile virus
dc.subjectantibody affinity
dc.subjectblood
dc.subjectgenetics
dc.subjectimmunology
dc.subjectreverse transcription polymerase chain reaction
dc.subjectseroepidemiology
dc.subjectWest Nile fever
dc.subjectWest Nile virus
dc.titleSerological screening of West Nile virus among blood donors in northern Cyprus
dc.typeArticle

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