In vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative

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dc.contributor.authorVural K.
dc.contributor.authorKosova F.
dc.contributor.authorKurt F.Ö.
dc.contributor.authorTuğlu İ.
dc.date.accessioned2024-07-22T08:10:23Z
dc.date.available2024-07-22T08:10:23Z
dc.date.issued2017
dc.description.abstractThe chaperone-binding drug, 17-allylamino-17-demethoxygeldanamycin, has recently come into clinical use. It is a derivative of geldanamycin, an ansamycin benzoquinone antibiotic with anti-carcinogenic effect. Understanding the effect of this drug on the cancer cells and their niche is important for treatment. We applied 17-allylamino-17-demethoxygeldanamycin to colon cancer cell line (Colo 205) on matrix molecules to investigate the relationship of apoptosis with terminal deoxynucleotidyl transferase dUTP nick end labeling immunocytochemistry and related gene expression. We used laminin and collagen I for matrix molecules and vascular endothelial growth factor for angiogenic structure. We also examined apoptosis-related signaling pathway including mitochondrial proteins, cytochrome c, Bcl-2, caspase-9, Apaf-1 expression using real-time polymerase chain reaction. There was clear effect of 17-allylamino-17-demethoxygeldanamycin that killed more cells on tissue culture plastic compared to matrix molecules. The IC50 value was 0.58 µg/mL for tissue culture plastic compared with 0.64 µg/mL for laminin and 0.75 µg/mL for collagen I. The analyses showed that more cells on matrix molecules underwent apoptosis compared to that on tissue culture plastic. Apoptosis-related gene expression was similar in which Bcl-2 expression decreased and proapoptotic gene expression of the cells on matrix molecules increased compared to that on tissue culture plastic. However, the application of 17-allylamino-17-demethoxygeldanamycin was more effective for the cells on collagen I compared to the cells on laminin. There was also a decrease in angiogenesis as shown by the vascular endothelial growth factor staining. This was more pronounced by coating of the tissue culture plastic with matrix molecules. Our results supported the anti-cancer effect of 17-allylamino-17-demethoxygeldanamycin, and this effect depended on matrix molecules. This effect occurs through apoptosis, and related genes were also altered. All these genes may serve for novel target under the effect of matrix substrate. However, correct interpretation of the results requires further studies. © 2017, © The Author(s) 2017.
dc.identifier.DOI-ID10.1177/1010428317720569
dc.identifier.issn10104283
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15211
dc.language.isoEnglish
dc.publisherSAGE Publications Ltd
dc.rightsAll Open Access; Gold Open Access
dc.subjectAnticarcinogenic Agents
dc.subjectApoptosis
dc.subjectApoptotic Protease-Activating Factor 1
dc.subjectBenzoquinones
dc.subjectCaspase 9
dc.subjectCell Line, Tumor
dc.subjectCollagen
dc.subjectColon
dc.subjectColonic Neoplasms
dc.subjectDNA Nucleotidylexotransferase
dc.subjectHumans
dc.subjectLactams, Macrocyclic
dc.subjectProto-Oncogene Proteins c-bcl-2
dc.subjectRifabutin
dc.subjectSignal Transduction
dc.subjectVascular Endothelial Growth Factor A
dc.subjectapoptotic protease activating factor 1
dc.subjectcaspase 9
dc.subjectcollagen type 1
dc.subjectcytochrome c
dc.subjectDNA nucleotidylexotransferase
dc.subjectlaminin
dc.subjectmitochondrial protein
dc.subjectprotein bcl 2
dc.subjecttanespimycin
dc.subjectvasculotropin
dc.subjectantineoplastic agent
dc.subjectapoptotic protease activating factor 1
dc.subjectbenzoquinone derivative
dc.subjectcaspase 9
dc.subjectcollagen
dc.subjectDNA nucleotidylexotransferase
dc.subjectgeldanamycin
dc.subjectmacrocyclic lactam
dc.subjectprotein bcl 2
dc.subjectquinone derivative
dc.subjectrifabutin
dc.subjecttanespimycin
dc.subjectvasculotropin A
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer resistance
dc.subjectcell function
dc.subjectcell killing
dc.subjectcell proliferation
dc.subjectCOLO 205 cell line
dc.subjectcolorectal cancer
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectdrug efficacy
dc.subjectdrug mechanism
dc.subjectgene expression regulation
dc.subjectgene targeting
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectIC50
dc.subjectimmunocytochemistry
dc.subjectin vitro study
dc.subjectintracellular signaling
dc.subjectoxidative stress
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectreal time polymerase chain reaction
dc.subjecttissue culture
dc.subjecttumor vascularization
dc.subjectTUNEL assay
dc.subjectupregulation
dc.subjectcolon
dc.subjectColonic Neoplasms
dc.subjectdrug effects
dc.subjectmetabolism
dc.subjectsignal transduction
dc.subjecttumor cell line
dc.titleIn vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative
dc.typeArticle

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