Enhanced cytotoxicity and apoptosis by thymoquinone in combination with zoledronic acid in hormone- and drugresistant prostate cancer cell lines

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dc.contributor.authorDirican A.
dc.contributor.authorErten C.
dc.contributor.authorAtmaca H.
dc.contributor.authorBozkurt E.
dc.contributor.authorKucukzeybek Y.
dc.contributor.authorVarol U.
dc.contributor.authorTarhan M.O.
dc.contributor.authorKaraca B.
dc.contributor.authorUslu R.
dc.date.accessioned2024-07-22T08:15:23Z
dc.date.available2024-07-22T08:15:23Z
dc.date.issued2014
dc.description.abstractPurpose: Thymoquinone (TQ), an active ingredient of black seed oil (Nigella Sativa), has been shown to possess cytotoxic activity against a variety of cancer cell lines. Our purpose was to investigate if the cytotoxic and apoptotic effect of zoledronic acid (ZA) can be enhanced by the addition of the TQ in hormone- and drug-refractory prostate cancer cells PC-3 and DU-145. Methods: XTT cell proliferation assay was used to assess cytotoxicity; DNA fragmentation and caspase 3/7 activity were also measured. Results: The combination of TQ and ZA resulted in a significant synergistic cytotoxic activity and DN A fragmentation when compared to any single agent alone, in a dose- and time-dependent manner. In addition, TQ and ZA combination increased the caspase 3/7 activity in PC-3 cell line, while this activity could not be demonstrated in DU-145 cell line. Conclusion: TQ and ZA had minimal hematological and non-hematological toxicity profile compared to cytotoxic agents. So, this combination may be an alternative approach for patients who are unable to be treated by conventional treatments because of poor performance status.
dc.identifier.issn11070625
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16727
dc.language.isoEnglish
dc.publisherZerbinis Publications
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectBenzoquinones
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectDiphosphonates
dc.subjectHumans
dc.subjectImidazoles
dc.subjectMale
dc.subjectProstatic Neoplasms
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectthymoquinone
dc.subjectzoledronic acid
dc.subjectantineoplastic agent
dc.subjectbenzoquinone derivative
dc.subjectbisphosphonic acid derivative
dc.subjectimidazole derivative
dc.subjectthymoquinone
dc.subjectzoledronic acid
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer resistance
dc.subjectcell viability
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectDNA fragmentation
dc.subjectdrug cytotoxicity
dc.subjectdrug potentiation
dc.subjectdrug resistance
dc.subjectdrug response
dc.subjectenzyme activity
dc.subjecthormone resistance
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC50
dc.subjectin vitro study
dc.subjectmale
dc.subjectprostate cancer
dc.subjectprostate cancer cell line
dc.subjectapoptosis
dc.subjectcell proliferation
dc.subjectprostate tumor
dc.subjecttumor cell line
dc.titleEnhanced cytotoxicity and apoptosis by thymoquinone in combination with zoledronic acid in hormone- and drugresistant prostate cancer cell lines
dc.typeArticle

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