In vitro cytotoxicity of magnetic-fluorescent bioactive glasses on SaOS-2, MC3T3-E1, BJ fibroblast cells, their hemolytic activity, and sorafenib release behavior

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dc.contributor.authorDeliormanlı A.M.
dc.contributor.authorRahman B.
dc.contributor.authorAtmaca H.
dc.date.accessioned2024-07-22T08:01:25Z
dc.date.available2024-07-22T08:01:25Z
dc.date.issued2024
dc.description.abstractIn the study, the fabrication of superparamagnetic-fluorescent bioactive glasses in the form of the particle, nanofiber, and 3D scaffolds was performed by including maghemite (γ-Fe2O3) nanoparticles and photoluminescent rare earth element ions (Eu3+, Gd3+, and Yb3+) using sol-gel, electrospinning, and robocasting techniques, respectively. The in vitro cytotoxicity of the magnetic-fluorescent bioactive glasses on osteosarcoma SaOS-2, pre-osteoblast MC3T3-E1, and BJ fibroblast cells, as well as their hemolytic activity and sorafenib tosylate loading and release behavior, were investigated. The cytotoxicity of the bioactive glass samples was tested using the MTT assay. Additionally, the alkaline phosphatase activity of the studied glasses was examined as a function of time. The mineralization behavior of the pre-osteoblast cell-seeded glass samples was analyzed using Alizarin red S staining. Results revealed that the in vitro cytotoxicity of the studied bioactive glasses in the form of particles and nanofibers depended on the sample concentration, whereas in the case of the 3D scaffolds, no cytotoxic response was observed on the osteosarcoma, pre-osteoblast, and fibroblast cells. Similarly, particle and nanofiber-based glass samples induced dose-dependent hemolysis on red blood cells. Drug loading rates were much lower for the 3D scaffolds compared to the particle and nanofiber-based samples. Drug release rates ranged from 25 % to 90 %, depending on the bioactive glass morphology and the pH of the release medium. It was concluded that the studied bioactive glasses have the potential to be used in tissue engineering applications and cancer therapy. © 2024 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.bioadv.2024.213782
dc.identifier.issn27729508
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11448
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectCellulose
dc.subjectColoring Agents
dc.subjectElliptocytosis 4
dc.subjectElliptocytosis, Hereditary
dc.subjectHemolysis
dc.subjectHumans
dc.subjectMagnetic Phenomena
dc.subjectOsteosarcoma
dc.subjectPhysical Phenomena
dc.subjectPoloxamer
dc.subjectSorafenib
dc.subjectAlkalinity
dc.subjectBioactive glass
dc.subjectBlood
dc.subjectCell culture
dc.subjectControlled drug delivery
dc.subjectCytotoxicity
dc.subjectFibroblasts
dc.subjectFluorescence
dc.subjectHematite
dc.subjectPhosphatases
dc.subjectRare earths
dc.subjectScaffolds (biology)
dc.subjectSol-gels
dc.subjectSulfur compounds
dc.subjectTargeted drug delivery
dc.subjectcellulose
dc.subjectcoloring agent
dc.subjectpoloxamer
dc.subjectpre-seed
dc.subjectsorafenib
dc.subject3D scaffolds
dc.subjectBJ cell
dc.subjectFibroblast cells
dc.subjectHaemolysis
dc.subjectIn-vitro
dc.subjectMC3T3-E1
dc.subjectSaOS-2
dc.subjectSorafenib
dc.subjectSorafenib tosylate
dc.subjectVitro cytotoxicities
dc.subjectelliptocytosis
dc.subjecthemolysis
dc.subjecthuman
dc.subjectmagnetism
dc.subjectosteosarcoma
dc.subjectphysical phenomena
dc.subjectNanofibers
dc.titleIn vitro cytotoxicity of magnetic-fluorescent bioactive glasses on SaOS-2, MC3T3-E1, BJ fibroblast cells, their hemolytic activity, and sorafenib release behavior
dc.typeArticle

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