Effects of Oxytocin on Glutamate Mediated Neurotoxicity in Neuroblastoma Cell Culture

Simple item page
dc.contributor.authorGürbüz Özgür B.
dc.contributor.authorVural K.
dc.contributor.authorTuğlu M.İ.
dc.date.accessioned2024-07-22T08:01:49Z
dc.date.available2024-07-22T08:01:49Z
dc.date.issued2024
dc.description.abstractIntroduction: We aimed to investigate the effects of oxytocin on neurite growth, cell viability, cell proliferation and apoptosis to demonstrate its neuroprotective effect on glutamate induced neurotoxicity in human neuroblastoma SH-SY5Y cell culture. Method: The effect of oxytocin on the toxic effects of glutamate in human neuroblastoma SH-SY5Y cell line with the Neurotoxicity Screening Test (NTT), apoptotic effects by Terminal Transferase dUTP Nick End Labeling (TUNEL) method and cell viability test by 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) method. In the NTT test; Neurotoxicity was induced by adding glutamate at a concentration of 32 µM to the cell culture. Oxytocin was added at 1, 3, 10, 30 and 100 µM concentrations and its effect on neurite elongation was investigated. It was demonstrated by TUNEL method that application of glutamate caused apoptosis. Afterwards, when glutamate and different doses of oxytocin were given, antiapoptotic effect was evaluated with the apoptotic index. Results: Glutamate was found to have a dose-dependent neurotoxic effect and reduced neurite elongation by 50% at a concentration of 32 μM. It was shown that the inhibition of neurite elongation caused by glutamate decreased in a dose-dependent manner by applying oxytocin. Especially oxytocin was found to significantly reduce neurite inhibition and show a neuroprotective effect starting from 10 µM concentrations. The concentration at which glutamate reduces cell proliferation by 50% was determined as 54 µM in MTT. Subsequently, it was observed that the adverse effect of glutamate on cell proliferation significantly decreased with oxytocin administration, depending on the dose. Conclusion: It was found that different concentrations of glutamate have a significant toxic effect on cell proliferation and viability, glutamate inhibits neurite elongation in a dose-dependent manner; oxytocin reduces neurite inhibition caused by glutamate, has a neuroprotective effect, increases cell viability and has antiapoptotic effects. © 2023 by Turkish Association of Neuropsychiatry.
dc.identifier.DOI-ID10.29399/npa.28377
dc.identifier.issn13000667
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11574
dc.language.isoEnglish
dc.publisherTurkish Neuropsychiatric Society
dc.rightsAll Open Access; Green Open Access
dc.subjectglutamic acid
dc.subjectoxytocin
dc.subjectantiapoptotic activity
dc.subjectapoptosis
dc.subjectapoptosis index
dc.subjectArticle
dc.subjectcell culture
dc.subjectcell growth
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcytotoxicity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunohistochemistry
dc.subjectlactation
dc.subjectMTT assay
dc.subjectnerve cell differentiation
dc.subjectneural stem cell
dc.subjectneurite
dc.subjectneurite outgrowth
dc.subjectneuroblastoma
dc.subjectneuroblastoma cell
dc.subjectneuroprotection
dc.subjectneurotoxicity
dc.subjectoxidative stress
dc.subjectprotein expression
dc.subjectSH-SY5Y cell line
dc.subjectTUNEL assay
dc.titleEffects of Oxytocin on Glutamate Mediated Neurotoxicity in Neuroblastoma Cell Culture
dc.typeArticle

Files

Collections

Scopus Koleksiyonu