Effect of nicotine on RANKL and OPG and bone mineral density

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dc.contributor.authorMizrak S.
dc.contributor.authorTuran V.
dc.contributor.authorInan S.
dc.contributor.authorUysal A.
dc.contributor.authorYilmaz C.
dc.contributor.authorErcan G.
dc.date.accessioned2024-07-22T08:14:52Z
dc.date.available2024-07-22T08:14:52Z
dc.date.issued2014
dc.description.abstractAim: The signaling pathway OPG/RANK/RANKL is a key in maintaining the balance between the activity of osteoblasts and osteoclasts in order to prevent bone loss. In this study, our aim was to assess the effects of long-term nicotine exposure on plasma RANKL and OPG levels, tissue RANKL and OPG immunoreactivities, and bone mineral density (BMD) scores in rats.; Materials and Methods: Thirty-six Swiss Albino rats weighing 70 ± 10 g were divided into three groups. While the controls (n = 12) were only given normal drinking water, for low-dose nicotine (LDN) group (n = 12) 0.4 mg/kg/day; for high-dose nicotine (HDN) group (n = 12), 6.0 mg/kg/day nicotine was added to drinking water for a year. At the end of 12th month, BMD scores were measured using an X-ray absorptiometry and bone turnover was assessed by measuring plasma RANKL and OPG levels and RANKL and OPG immunoreactivities in tail vertebrae of the rats.; Results: There was no statistically significant difference in BMD scores of lumbar spine and femoral regions of the nicotine groups in comparison to controls. Plasma OPG levels were found to be significantly higher in HDN group, in comparison to the controls and LDN groups (p = .001) unlike plasma RANKL levels. Tissue RANKL and OPG immunoreactivities decreased significantly in the LDN and HDN groups (p < .001, p < .01, respectively). Conclusions: The results of this study show that nicotine is not primarily responsible for the decrease in BMD frequently seen in smokers. Measuring plasma RANKL and OPG levels did not reflect tissue immunoreactivities. Copyright © 2014 Informa Healthcare USA, Inc.
dc.identifier.DOI-ID10.3109/08941939.2014.916369
dc.identifier.issn08941939
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16665
dc.language.isoEnglish
dc.publisherInforma Healthcare
dc.subjectAbsorptiometry, Photon
dc.subjectAdministration, Oral
dc.subjectAnimals
dc.subjectBone Density
dc.subjectDose-Response Relationship, Drug
dc.subjectFemale
dc.subjectMale
dc.subjectModels, Animal
dc.subjectNicotine
dc.subjectOsteoprotegerin
dc.subjectRANK Ligand
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectRisk Factors
dc.subjectSpine
dc.subjectcotinine
dc.subjectnicotine
dc.subjectosteoclast differentiation factor
dc.subjectosteoprotegerin
dc.subjectnicotine
dc.subjectosteoclast differentiation factor
dc.subjectosteoprotegerin
dc.subjectTnfrsf11b protein, rat
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbone density
dc.subjectbone turnover
dc.subjectcontrolled study
dc.subjectdual energy X ray absorptiometry
dc.subjectfemale
dc.subjectfemur
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectlong term exposure
dc.subjectlumbar spine
dc.subjectmale
dc.subjectnonhuman
dc.subjectosteolysis
dc.subjectprotein blood level
dc.subjectrat
dc.subjectrisk factor
dc.subjectscoring system
dc.subjectsmoking
dc.subjecttissue level
dc.subjectanimal
dc.subjectbone density
dc.subjectdose response
dc.subjectdrug effects
dc.subjectinbred rat strain
dc.subjectmetabolism
dc.subjectoral drug administration
dc.subjectphoton absorptiometry
dc.subjectspine
dc.titleEffect of nicotine on RANKL and OPG and bone mineral density
dc.typeArticle

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