The effect of acid adaptation conditions on heat resistance of Escherichia coli O157: H7

Simple item page
dc.contributor.authorTosun H.
dc.contributor.authorGönül Ş.A.
dc.date.accessioned2024-07-22T08:23:37Z
dc.date.available2024-07-22T08:23:37Z
dc.date.issued2005
dc.description.abstractThe objective of this study was to determine the effect of acid adaptation conditions on heat resistance of E. coli O157: H7 932. E. coli O157: H7 was adapted to acid by exposing the cells to pH 4.5 (2h), pH 5.0 (1h), and pH 5.5 (1 h) in tryptic soy broth. D and z values of the acid adapted and control cultures at 54°C, 56°C, and 58°C were determined in E buffer. The heat resistance of E. coli O157: H7 increased significantly (p<0.05) after acid adaptation at pH 4.5 or pH 5.0. E. coli O157: H7 adapted to acid at pH 4.5 for 2 h had the highest D values at all temperatures tested (20.3-10.7-3.3 min) while D values of culture adapted to acid at pH 5.0 for 1 h were 18.2, 7.9, and 2.6 min at 54°C, 56°C and 58°C, respectively. Heat resistance of culture adapted to acid at pH 5.5 for 1 h and the control culture was not significantly different (P<0.05). Culture adapted to acid at pH 4.5 had the highest z value (5.10°C), whereas control culture had the lowest z value (4.33°C). This study showed that the magnitude of heat tolerance changed with the adaptation pH and at low adaptation pH, E. coli O157: H7 showed maximum heat resistance. Acid adaptation at pH 4.5 or 5.0 provides E. coli O157: H7 with cross-protection against heat treatments, and that this factor must be considered to estimate this pathogen's thermal tolerance accurately.
dc.identifier.issn17331331
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19615
dc.language.isoEnglish
dc.subjectAdaptation, Physiological
dc.subjectEscherichia coli O157
dc.subjectFood Microbiology
dc.subjectHeat
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectBacteria (microorganisms)
dc.subjectEscherichia coli
dc.subjectEscherichia coli O157:H7
dc.subjectacid
dc.subjectacid tolerance
dc.subjectadaptation
dc.subjectarticle
dc.subjectbacterial survival
dc.subjectbacterium culture
dc.subjectcontrolled study
dc.subjectEscherichia coli O157
dc.subjectheat tolerance
dc.subjectheat treatment
dc.subjectnonhuman
dc.subjectpH
dc.titleThe effect of acid adaptation conditions on heat resistance of Escherichia coli O157: H7
dc.typeArticle

Files

Collections

Scopus Koleksiyonu