The Effect of N-Acetylcysteine on the Neurofilament and Nerve Growth Factor Expression after Gentamicin Induced Cochlear Damage; [Gentamisin ile Oluşturulan Kohlear Hasar üzerine N-Asetilsisteinin Nörofilament ve Sinir Büyüme Faktörü Salınımına Etkisi]

Sarsmaz H.Y.; Gürgen S.G.; Somdaş M.A.

The Effect of N-Acetylcysteine on the Neurofilament and Nerve Growth Factor Expression after Gentamicin Induced Cochlear Damage; [Gentamisin ile Oluşturulan Kohlear Hasar üzerine N-Asetilsisteinin Nörofilament ve Sinir Büyüme Faktörü Salınımına Etkisi]

Date

2022

Authors

Sarsmaz H.Y.

Gürgen S.G.

Somdaş M.A.

Publisher

Gazi Universitesi

Abstract

Objective: To investigate of the effectiveness of N-acetylcysteine (NAC) in preventing cochlear damage due to gentamicin by the means of expression of neuronal factors Metods: In our study, 36 female Spraguea Dawley rats were used. They were divided into 3 groups such as (saline), Gentamicin + Serum physiological (saline) and Gentamicin + NAC. After 15 days, the rats were sacrificed. Cochleae were removed and examined histopathologically. The immunoreactivity of neurofilament and neuronal growth factor primary antibodies was examined in the tissues taken. Results: Neuroflament immunoreactivity was quite significant in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, whereas it was weak in the Gentamicin + serum physiological group. Also neuronal growth factor immunoreactivity was moderate in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, it was negative at vestibular ganglia, and weak in afferent and efferent nerve fiber bundles in the Gentamicin + serum physiological group. Conclusion: It was shown immunohistochemically that ototoxicity caused by gentamicin was significantly reduced when the NAC combination was applied. © Copyright 2022 by Gazi University Medical Faculty