Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line

Oztatlici M.; Ozdemir A.T.; Oztatlici H.; Kucukhuyuk S.; Ozgul Ozdemir R.B.; Orhan H.; Ozbilgin K.

Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line

Date

2024

Authors

Publisher

Kare Publishing

Abstract

Objectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB-231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co-cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro-inflammatory and anti-inflammatory cy-tokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co-culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro-inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV-treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. Moreover, TNF-α and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in-vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies. © 2024 by Eurasian Journal of Medicine and Oncology.