Effect of erythropoietin on acoustically traumatized rat cochlea an immunohistochemical study
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Date
2014
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Abstract
Objective: To investigate the audiological and histopathological effects of erythropoietin on acoustic overstimulation in rats. Study design: Twenty-two male Wistar albino rats were divided into 3 groups: sham group (n=7), erythropoietin injection group (n = 8), and saline injection group (n=7). Both erythropoietin and saline injection groups were exposed to white noise (100 decibel [dB] sound pressure level [SPL]) for 3 hours. Auditory brainstem responses were measured before, immediately after, and on the 7th day of noise exposure. All animals were sacrificed on the 7th day and temporal bones were collected. The serial sections of the cochleae were stained by caspase-3 and caspase-9 immunostaining and by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method in order to detect apoptotic cells. Results: In the saline group statistically significant differences were detected between the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli. However, when the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli were compared in the erythropoietin injection group, no statistically significant difference was determined. Histopathologic evaluations demonstrated that erythropoietin decreased the amount of apoptotic cells in the cochlea. Conclusion: Erythropoietin is likely to prevent the acute threshold changes and decrease the amount of apoptosis in cochlea after acoustic overstimulation in rats. © Science Printers and Publishers, Inc.
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Animals , Apoptosis , Cochlea , Erythropoietin , Evoked Potentials, Auditory, Brain Stem , Hearing Loss, Noise-Induced , Immunohistochemistry , Male , Rats , Animalia , Rattus , caspase 3 , caspase 9 , erythropoietin , erythropoietin , agents acting on the auditory and vestibular systems , caspase 3 , caspase 9 , protective agent , recombinant erythropoietin , animal experiment , animal tissue , apoptosis , Article , cochlea injury , controlled study , Corti organ , evoked brain stem response , histopathology , immunohistochemistry , male , nick end labeling , noise injury , nonhuman , rat , sound pressure , spiral ganglion , spiral ligament , temporal bone , white noise , animal , cochlea , drug effects , evoked brain stem auditory response , Hearing Loss, Noise-Induced , injuries , pathology , adult , animal cell , animal model , audiology , auditory stimulation , auditory threshold , brain stem response , cochlea injury , histopathology , noise injury , priority journal , protein expression , sensory nerve cell , TUNEL assay