The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway

Simple item page
dc.contributor.authorBecer E.
dc.contributor.authorHanoğlu D.Y.
dc.contributor.authorKabadayı H.
dc.contributor.authorHanoğlu A.
dc.contributor.authorVatansever S.
dc.contributor.authorYavuz D.
dc.contributor.authorMeriçli F.
dc.contributor.authorMeriçli A.H.
dc.date.accessioned2024-07-22T08:08:50Z
dc.date.available2024-07-22T08:08:50Z
dc.date.issued2019
dc.description.abstractObjective: Colchicum pusillum belongs to the family Colchicaceae that particularly rich in tropolonic alkaloids. The aim of this study was to investigate the cytotoxicity and in vitro anticancer activity of Colchicum pusillum ethanolic extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Materials and methods: Colchicum pusillum was collected and extracted with ethanol. Different concentrations of Colchicum pusillum extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assays. Anticancer and antiproliferative activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to β-catenin, Ki-67, LGR-5 Ki-67, DKK1, Frizzled-4, Wnt4, Wnt7a and caspase3 in Colo-741 cells. Results: All concentrations of Colchicum pusillum extract had toxic effect in Colo-320 cells. Because of this, we used Colchicum pusillum extract at 20 μg/ml for evaluate anticancer activities only in Colo-741 cells. As a result of immunohistochemical staining, β-catenin, LGR-5 and caspase-3 immunoreactivities were significantly increased while Wnt7a immunostaining intensity was decreased in Colo-741 cells. Conclusion We conclude that Colchicum pusillum extract increased β-catenin and LGR-5 via Wnt/β-catenin pathway in colon cancer cells. Interestingly, it decreased other signaling molecule, Wnt7a which is assumed to play protective role during carcinogenesis. Also, it increased significantly caspase-3 immunoreactivity showing that apoptotic pathways were triggered. © 2018
dc.identifier.DOI-ID10.1016/j.gene.2018.11.047
dc.identifier.issn03781119
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14556
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAdenocarcinoma
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectbeta Catenin
dc.subjectCell Line, Tumor
dc.subjectColchicum
dc.subjectColonic Neoplasms
dc.subjectHumans
dc.subjectNeoplasm Proteins
dc.subjectPlant Extracts
dc.subjectWnt Signaling Pathway
dc.subjectalcohol
dc.subjectantineoplastic agent
dc.subjectbeta catenin
dc.subjectcaspase 3
dc.subjectColchicum pusillum extract
dc.subjectdickkopf 1 protein
dc.subjectG protein coupled receptor
dc.subjectKi 67 antigen
dc.subjectleucine rich repeat containing G protein coupled receptor 5
dc.subjectplant extract
dc.subjectsecreted frizzled related protein 4
dc.subjectunclassified drug
dc.subjectWnt protein
dc.subjectWnt4 protein
dc.subjectWnt7a protein
dc.subjectantineoplastic agent
dc.subjectbeta catenin
dc.subjectplant extract
dc.subjecttumor protein
dc.subjectantineoplastic activity
dc.subjectantiproliferative activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer cell
dc.subjectColchicum
dc.subjectColchicum pusillum
dc.subjectCOLO 320 cell line
dc.subjectColo 741 cell line
dc.subjectcolon adenocarcinoma cell line
dc.subjectcolon cancer
dc.subjectcolon cancer cell line
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC50
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectin vitro study
dc.subjectMTT assay
dc.subjectpriority journal
dc.subjectWnt signaling
dc.subjectadenocarcinoma
dc.subjectchemistry
dc.subjectcolon tumor
dc.subjectdrug effect
dc.subjectmetabolism
dc.subjectpathology
dc.subjecttumor cell line
dc.subjectWnt signaling
dc.titleThe effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway
dc.typeArticle

Files

Collections

Scopus Koleksiyonu