Zoledronic acid effects interleukin-6 expression in hormone-independent prostate cancer cell lines
| dc.contributor.author | Asbagh L.A. | |
| dc.contributor.author | Uzunoglu S. | |
| dc.contributor.author | Cal C. | |
| dc.date.accessioned | 2024-07-22T08:22:30Z | |
| dc.date.available | 2024-07-22T08:22:30Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | Objective: To investigate the inhibitory effects of zoledronic acid (ZA) on tumor related growth factor IL-6 in hormone resistant prostate cancer cell lines. The association between apoptosis and IL-6 inhibition was also assessed. Materials and Methods: PC-3 and DU145 cell lines were treated with different concentrations of ZA (1-100μM) at various intervals (24-72 h.). The cell viability was investigated by XTT assay and apoptotic effect was evaluated by cell death detection ELISA kit. Caspase 3/7 activity assay was performed to confirm apoptosis. IL-6 levels were measured by ELISA in the supernatant, and these data were also confirmed by IL-6 mRNA analysis using RT-PCR. Results: PC-3 and DU145 cell lines were sensitive to ZA mediated cytotoxicity in a dose- and time-dependent manner. However, the apoptotic effect was significantly different among PC-3 and DU145 cells (p < 0.05). IL-6 secretion was significantly lower in both cell lines, compared to the untreated control cells (p < 0.05). Although the increased inhibition of IL-6 secretion was associated with increased apoptosis in DU145 cells (p = 0.002), there was no similar association for PC-3 cell line (p = 0.347). When compared to the untreated controls, the number of cDNA copies was significantly lower in the ZA treated DU145 cell line at doses of 30 and 90μM (p < 0.05), suggesting a reduced expression of IL-6 mRNA. Conclusion: ZA exhibited a time- and dose-dependent apoptotic effect on PC-3,and DU145 prostate cancer cell lines and this effect was associated with inhibited secretion of IL-6 in DU145 cell line. | |
| dc.identifier.DOI-ID | 10.1590/s1677-55382008000300013 | |
| dc.identifier.issn | 16775538 | |
| dc.identifier.uri | http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19094 | |
| dc.language.iso | English | |
| dc.publisher | Brazilian Society of Urology | |
| dc.rights | All Open Access; Gold Open Access; Green Open Access | |
| dc.title | Zoledronic acid effects interleukin-6 expression in hormone-independent prostate cancer cell lines | |
| dc.type | Article |