Histopathological and ultrastructural effects of Losartan on embryonic rat kidney

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dc.contributor.authorAkil I.
dc.contributor.authorInan S.
dc.contributor.authorGurcu B.
dc.contributor.authorNazikoglu A.
dc.contributor.authorOzbilgin K.
dc.contributor.authorMuftuoglu S.
dc.date.accessioned2024-07-22T08:23:40Z
dc.date.available2024-07-22T08:23:40Z
dc.date.issued2005
dc.description.abstractThe aim of our study was to investigate the histopathological, immunohistochemical and ultrastructural effects of Losartan (a selective angiotensin II type-1 receptor blocker) on renal development in rats. Twelve pregnant rats were divided into control and experimental groups. In the experimental group, Losartan (10 mg/kg/day) was given via nasogastric tube, between the sixth day of implantation and time of sacrifice on embryonic days 18 and 20. All formalin-fixed, paraffin wax-embedded renal tissue sections were stained with hematoxylin and eosin or labelled for binding of primary antibodies against transforming growth factor-β (TGF-β 1,-2,-3) using an avidin-biotin-peroxidase method. For electron microscopic examination, samples were fixed with glutaraldehyde and osmium tetroxide and embedded in araldite. Glomerular basement membrane (GBM) thickness was measured and compared using an unpaired t-test. Angiotensin II type-1 receptor antagonism by Losartan inhibited renal growth and delayed nephron maturation. Increased immunoreactivity of TGF-β's was observed in developing nephron precursors and interstitial cells in the experimental group. Electron microscopical examination showed that thickening of the GBM was normal in the control group but an irregular thickening was seen in the experimental group (p<0.001). It was also seen that epithelial cells of developing tubules underwent apoptosis in the experimental group. Thus, renal development in rats seems to depend on an intact renin-angiotensin system. © 2005 Elsevier GmbH. All rights reserved.
dc.identifier.DOI-ID10.1016/j.acthis.2005.06.011
dc.identifier.issn00651281
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19647
dc.language.isoEnglish
dc.publisherElsevier GmbH
dc.subjectAnimalia
dc.subjectBiological organs
dc.subjectCells
dc.subjectElectron microscopy
dc.subjectEnzymes
dc.subjectMicroscopic examination
dc.subjectPathology
dc.subjectVitamins
dc.subjectangiotensin 2 receptor antagonist
dc.subjectavidin
dc.subjectbiotin
dc.subjectformaldehyde
dc.subjectglutaraldehyde
dc.subjectlosartan
dc.subjectosmium tetraoxide
dc.subjectparaffin
dc.subjecttransforming growth factor beta1
dc.subjecttransforming growth factor beta2
dc.subjecttransforming growth factor beta3
dc.subjectHistopathology
dc.subjectLosartan
dc.subjectRenal development
dc.subjectTGF-β
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcell ultrastructure
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectembryo
dc.subjectembryo development
dc.subjectembryonal tissue
dc.subjectepithelium cell
dc.subjectglomerulus basement membrane
dc.subjecthistopathology
dc.subjectimmunoreactivity
dc.subjectkidney
dc.subjectkidney development
dc.subjectkidney epithelium
dc.subjectkidney parenchyma
dc.subjectLeydig cell
dc.subjectmeasurement
dc.subjectnephron
dc.subjectnephrotoxicity
dc.subjectnonhuman
dc.subjectrat
dc.subjectthickness
dc.subjecttoxicokinetics
dc.subjectHistology
dc.titleHistopathological and ultrastructural effects of Losartan on embryonic rat kidney
dc.typeArticle

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