Investigation of cytotoxic and apoptotic effects of disodium pentaborate decahydrate on ovarian cancer cells and assessment of gene profiling

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dc.contributor.authorGunel N.S.
dc.contributor.authorYildirim N.
dc.contributor.authorOzates N.P.
dc.contributor.authorOktay L.M.
dc.contributor.authorBagca B.G.
dc.contributor.authorSogutlu F.
dc.contributor.authorOzsaran A.
dc.contributor.authorKorkmaz M.
dc.contributor.authorBiray Avci C.
dc.date.accessioned2024-07-22T08:03:45Z
dc.date.available2024-07-22T08:03:45Z
dc.date.issued2023
dc.description.abstractAfter revealing the anti-cancer properties of boron, which is included in the category of essential elements for human health by the World Health Organization, the therapeutic potential of boron compounds has been begun to be evaluated, and its molecular effect mechanisms have still been among the research subjects. In ovarian cancer, mutations or amplifications frequently occur in the PI3K/Akt/mTOR pathway components, and dysregulation of this pathway is shown among the causes of treatment failure. In the present study, it was aimed to investigate the anti-cancer properties of boron-containing DPD in SKOV3 cells, which is an epithelial ovarian cancer model, through PI3K/AKT/mTOR pathway. The cytotoxic activity of DPD in SKOV3 cells was evaluated by WST-1 test, apoptotic effect by Annexin V and JC-1 test. The gene expressions associated with PI3K/AKT/mTOR pathway were determined by real-time qRT-PCR. In SKOV3 cells, the IC50 value of DPD was found to be 6.7 mM, 5.6 mM, and 5.2 mM at 24th, 48th and 72nd hour, respectively. Compared with the untreated control group, DPD treatment was found to induce apoptosis 2.6-fold and increase mitochondrial membrane depolarization 4.5-fold. DPD treatment was found to downregulate PIK3CA, PIK3CG, AKT2, IGF1, IRS1, MAPK3, HIF-1, VEGFC, CAB39, CAB39L, STRADB, PRKAB2, PRKAG3, TELO2, RICTOR, MLST8, and EIF4B genes and upregulate TP53, GSK3B, FKBP8, TSC2, ULK1, and ULK2 genes. These results draw attention to the therapeutic potential of DPD, which is frequently exposed in daily life, in epithelial ovarian cancer and show that it can be a candidate compound in combination with chemotherapeutics. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
dc.identifier.DOI-ID10.1007/s12032-022-01870-1
dc.identifier.issn13570560
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12431
dc.language.isoEnglish
dc.publisherSpringer
dc.rightsAll Open Access; Green Open Access
dc.subjectAntigens, Neoplasm
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectApoptosis Regulatory Proteins
dc.subjectBoron
dc.subjectCarcinoma, Ovarian Epithelial
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectFemale
dc.subjectHumans
dc.subjectOvarian Neoplasms
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectSignal Transduction
dc.subjectTOR Serine-Threonine Kinases
dc.subjectapoptosis inducing factor
dc.subjectboron derivative
dc.subjectcytostatic agent
dc.subjectcytotoxic agent
dc.subjectdisodium pentaborate decahydrate
dc.subjectglycogen synthase kinase 3beta
dc.subjecthypoxia inducible factor 1
dc.subjectinitiation factor 4B
dc.subjectinsulin receptor substrate 1
dc.subjectlipocortin 5
dc.subjectmammalian target of rapamycin
dc.subjectmitogen activated protein kinase 3
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein kinase B
dc.subjectprotein kinase B beta
dc.subjectprotein p53
dc.subjectrapamycin-insensitive companion of mTOR
dc.subjectserine threonine protein kinase ULK1
dc.subjectsomatomedin C
dc.subjecttarget of rapamycin complex subunit LST8
dc.subjecttuberin
dc.subjectunclassified drug
dc.subjectvasculotropin C
dc.subjectantineoplastic agent
dc.subjectapoptosis regulatory protein
dc.subjectboron
dc.subjectCAB39L protein, human
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein kinase B
dc.subjectsodium pentaborate
dc.subjecttarget of rapamycin kinase
dc.subjecttumor antigen
dc.subjectAkt signaling
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcell membrane depolarization
dc.subjectcontrolled study
dc.subjectcytostasis
dc.subjectdown regulation
dc.subjectdrug cytotoxicity
dc.subjectfemale
dc.subjectgene expression
dc.subjectgene expression profiling
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC50
dc.subjectmitochondrial membrane
dc.subjectovary cancer
dc.subjectproapoptotic activity
dc.subjectreal time reverse transcription polymerase chain reaction
dc.subjectSK-OV-3 cell line
dc.subjectupregulation
dc.subjectWST-1 assay
dc.subjectcell proliferation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectovary tumor
dc.subjectsignal transduction
dc.subjecttumor cell line
dc.titleInvestigation of cytotoxic and apoptotic effects of disodium pentaborate decahydrate on ovarian cancer cells and assessment of gene profiling
dc.typeArticle

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