The determination of physiological and DNA changes in seedlings of maize (Zea mays L.) seeds exposed to the waters of the Gediz River and copper heavy metal stress
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2015
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Abstract
In this study, the effects of the heavy metal-polluted waters of the Gediz River, which flow into the Aegean Sea, and different concentrations of copper (Cu) solutions on maize (Zea mays L.) seedlings are investigated with physiological parameters and random amplified polymorphic DNA (RAPD) assay. Results displayed physiologically a significant difference in root and stem length between the control seedlings and the seedlings grown with the waters of the Gediz River. Also, the certain ascending concentrations of copper solution (80, 160, 320, 640, and 1280 ppm) caused a significant decrease in root and stem length of seedlings compared to the control seedlings. As a result of the waters of the Gediz River and copper solution treatment, the changes occurred in RAPD profiles of seedlings observed as variations like increment and/or loss of bands compared with the control seedlings. And these changes were reflected as a decrease in genomic template stability (GTS, changes in RAPD profile) derived by genotoxicity. RAPD band profiles and GTS values showed consistent results with physiological parameter. In conclusion, the study revealed the environmental risk and negative effect of waters of the Gediz River on maize seedlings and the suitability of RAPD assay for the detection of environmental toxicology. © 2015, Springer International Publishing Switzerland.
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Copper , DNA , DNA Damage , Environmental Monitoring , Metals, Heavy , Plant Roots , Random Amplified Polymorphic DNA Technique , Rivers , Seedling , Seeds , Stress, Physiological , Turkey , Water , Water Pollutants, Chemical , Zea mays , Gediz River , Turkey , Zea mays , Copper , Grain (agricultural product) , Heavy metals , Physiological models , Physiology , Plants (botany) , Rivers , copper , water , copper , DNA , Metals, Heavy , Water , Water Pollutants, Chemical , Environmental toxicology , Gediz River , Genomic template stability , Genotoxicities , Maize (Zea mays L.) , Physiological parameters , Random amplified polymorphic dnas , RAPD , copper , DNA , environmental risk , genotoxicity , maize , physiological response , pollution exposure , river water , seedling , water pollution , Article , comparative study , concentration (parameters) , concentration response , controlled study , genetic stability , genomic template stability , genotoxicity , maize , nonhuman , plant seed , random amplified polymorphic DNA , river , root length , seedling , stem elongation , water pollution , chemistry , DNA damage , drug effects , environmental monitoring , physiology , Plant Roots , Random Amplified Polymorphic DNA Technique , Rivers , Seedling , Seeds , Stress, Physiological , toxicity , Turkey , Water Pollutants, Chemical , Zea mays , River pollution