Comparison of Löwenstein Jensen medium and BACTEC 460TB culture system for diagnosis of tuberculosis; [Tüberküloz tanısında Löwenstein Jensen besiyeri ile BACTEC 460TB kültür sisteminin karşılaştırılması]
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2010
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Objective: In this study, it was intestigated that whether the use of Löwenstein Jensen (LJ) medium with BACTEC460 TB liquid culture system contributed to bacteria isolation rates for diagnosis of tuberculosis. Material and Methods: A total of 4237 specimens were evaluated in the study for the results of microscopic examination and culture. Of 4237 specimens, 2719 were obtained from respiratory tract and remaining 1518 were obtained out of the respiratory tract. Results: According to the results of culture, Mycobacterium tuberculosis complex were isolated in 271 (6.4%) specimens on BACTEC 460TB system and in 238 (5.6%) specimens on Löwenstein Jensen medium. Contamination rates at BACTEC 460TB system and Löwenstein Jensen medium were 3.6% and 10.2%, respectively. The mean times of recovery were determined as 9.6 days for BACTEC system and 21 days for Löwenstein Jensen medium. When contaminated specimens were excluded, Mycobacterium tuberculosis was grown on at least one culture technique in 258 out of 3718 (6.9%) specimens. Sensitivity of BACTEC 460 TB culture system was found as 96%, while sensitivity of Löwenstein Jensen medium was found as 92%. The correlation between two culture techniques was found as 99%. Contribution of BACTEC 460 TB system and LJ medium alone to culture positivity were found as 8.3% and 3.9% respectively. No statistically significant difference was found when microscopic evaluation and culture systems were compared. Conclusion: It was concluded that the liquid culture systems are needed for rapid detection of tuberculosis, however liquid and solid culture systems should be used together in order to reach maximum isolation rates. © 2010 by Türkiye Klinikleri.
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agar medium , article , bacterial growth , bacterium culture , bacterium isolation , contamination , controlled study , human , intermethod comparison , liquid culture , loewenstein medium , major clinical study , microscopy , Mycobacterium tuberculosis , nonhuman , respiratory system , sensitivity and specificity , tuberculosis