Apoptotic and autophagic characteristics of perivisceral fat body remodeling of the greater wax moth Galleria mellonella and effects of juvenile hormone analog, fenoxycarb, on these processes

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dc.contributor.authorPoyraz Tinartas E.
dc.contributor.authorGoncu E.
dc.contributor.authorKoc K.
dc.date.accessioned2024-07-22T08:05:57Z
dc.date.available2024-07-22T08:05:57Z
dc.date.issued2021
dc.description.abstractIn holometabolous insects, many tissues and organs such as the fat body and midgut undergo a remodeling process during metamorphosis. Larval fat body cells are eliminated by programmed cell death (PCD), while tissue cells that adapt to adult life are formed by stem cells. In this study, we analyzed the features of the remodeling period of Galleria mellonella fat body in terms of PCD types, apoptotic and autophagic cell death characteristics. Besides, the effects of juvenile hormone (JH) on these processes were evaluated under the modified hormonal conditions via applications of JH analog, fenoxycarb. Several hallmarks of apoptotic and autophagic cell death were analyzed by morphological, biochemical, and molecular methods. The results of the present study have ascertained that the degeneration process of larval cells occurs via autophagic cell death accompanied by caspase-3 activity during the pupal period and it is regulated by 20-hydroxyecdysone (20HE) mediated by ecdysone receptor B1 (EcR-B1). Increased activity of the acid phosphatase and upregulation of ATG6 and ATG8 in parallel with the formation of autophagosomes in the fat body of Galleria during the pupal period strongly indicated that autophagy was the key player in the remodeling processes. © 2021 Wiley Periodicals LLC
dc.identifier.DOI-ID10.1002/arch.21780
dc.identifier.issn07394462
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13318
dc.language.isoEnglish
dc.publisherJohn Wiley and Sons Ltd
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectFat Body
dc.subjectInsecticides
dc.subjectJuvenile Hormones
dc.subjectLarva
dc.subjectMetamorphosis, Biological
dc.subjectMoths
dc.subjectPhenylcarbamates
dc.subjectPupa
dc.subjectcarbamic acid derivative
dc.subjectfenoxycarb
dc.subjectinsecticide
dc.subjectjuvenile hormone
dc.subjectanimal
dc.subjectapoptosis
dc.subjectautophagy
dc.subjectbody fat
dc.subjectdrug effect
dc.subjectlarva
dc.subjectmetamorphosis
dc.subjectmoth
dc.subjectphysiology
dc.subjectpupa
dc.titleApoptotic and autophagic characteristics of perivisceral fat body remodeling of the greater wax moth Galleria mellonella and effects of juvenile hormone analog, fenoxycarb, on these processes
dc.typeArticle

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