Two oestrous cycles. Ten days insulin treatment reduced ovarian leptin expression of rat

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dc.contributor.authorOzbilgin K.
dc.contributor.authorKuscu N.K.
dc.date.accessioned2024-07-22T08:23:48Z
dc.date.available2024-07-22T08:23:48Z
dc.date.issued2005
dc.description.abstractObjective: Previous studies have reported a role for insulin in leptin secretion from rat ovarian cells although the time dependent effect of insulin has yet to be obtained. The study aim is to determine the time dependent effect of insulin on leptin staining intensity in the rat ovary. Methods: Normally cycling rats were investigated in a spontaneous cycle (control, n=6), one cycle with insulin treatment 2U/day for 5 days (D5) (n=6), and 2 cycles with insulin treatment 2 U/day for 10 days (D10) (n=6) in the Department of Histology and Embryology, Celal Bayar University, Manisa, Turkey, from May 2004 to August 2004. Histological structures and leptin staining of the ovarian cells were investigated with immunohistochemical technique and evaluated by a semi-quantitative scoring system. Results: Number of follicles and stromal cells decreased with longer insulin treatment. No leptin staining was observed in granulosa cells of all groups. Staining intensity and H-score of the controls, D5 and D1O groups were (+++)/204, (++)/80 and (+)/9.5 for theca externa cells; (+++)/289.5, (++)/126 and (+)/65 for stroma; (++)/140, (++)/70 and (+)/21 for corpus luteum. The difference between H-scores of control and D1O groups was statistically significant in all tissue types (P<0.0 1). Conclusion: Insulin treatment for 10 days (2 estrous cycles) reduced leptin-staining intensity in various tissues of the rat ovary and decreased follicle development in a time dependent manner.
dc.identifier.issn03795284
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19688
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectEstrous Cycle
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectInsulin
dc.subjectLeptin
dc.subjectOvary
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectinsulin
dc.subjectleptin
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectcorpus luteum
dc.subjectestrus cycle
dc.subjectgene expression
dc.subjectgranulosa cell
dc.subjecthistology
dc.subjecthormone release
dc.subjectimmunohistochemistry
dc.subjectinsulin treatment
dc.subjectnonhuman
dc.subjectovary cell
dc.subjectovary follicle cell
dc.subjectovary follicle development
dc.subjectquantitative analysis
dc.subjectrat
dc.subjectscoring system
dc.subjectstatistical significance
dc.subjectstroma cell
dc.subjecttheca cell
dc.subjectTurkey (republic)
dc.subjectuniversity
dc.titleTwo oestrous cycles. Ten days insulin treatment reduced ovarian leptin expression of rat
dc.typeArticle

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